Kántor Orsolya, Varga Alexandra, Tóth Róbert, Énzsöly Anna, Pálfi Emese, Kovács-Öller Tamás, Nitschke Roland, Szél Ágoston, Székely Andrea, Völgyi Béla, Négyessy László, Somogyvári Zoltán, Lukáts Ákos
Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58, H-1094, Budapest, Hungary.
Department of Ophthalmology, Semmelweis University, Budapest, H-1085, Hungary.
Cell Tissue Res. 2015 Feb;359(2):409-421. doi: 10.1007/s00441-014-2047-x. Epub 2014 Nov 20.
Tissue non-specific alkaline phosphatase (TNAP), an abundant ectophosphatase, is present in various organs including the brain and retina of several vertebrate species. Evidence is emerging that TNAP influences neural functions in multiple ways. In rat, strong TNAP activity has been found in retinal vessels, photoreceptors, and both synaptic layers. In the present study, we identified eleven strata of the inner plexiform layer (IPL) by using TNAP histochemistry alone. The TNAP strata corresponded exactly to the strata seen after combined immunohistochemistry with four canonical IPL markers (TH-ChAT-CR-PKCα). Therefore, as described in other mammalian species, our data support the existence of multiple morphologically and functionally discernible IPL strata in rats. Remarkably, the stratification pattern of the IPL was severely disrupted in a diabetic rat model, even before changes in the canonical IPL markers were detectable. These findings indicate that TNAP histochemistry offers a more straightforward, but also more sensitive, method for investigating retinal strata and their diabetes-induced degeneration.
组织非特异性碱性磷酸酶（TNAP）是一种丰富的胞外磷酸酶，存在于包括几种脊椎动物的大脑和视网膜在内的各种器官中。越来越多的证据表明，TNAP以多种方式影响神经功能。在大鼠中，已在视网膜血管、光感受器以及两个突触层中发现强烈的TNAP活性。在本研究中，我们仅使用TNAP组织化学就鉴定出了内网状层（IPL）的11个分层。TNAP分层与使用四种典型IPL标记物（TH - ChAT - CR - PKCα）进行联合免疫组织化学后看到的分层完全一致。因此，正如在其他哺乳动物物种中所描述的那样，我们的数据支持大鼠中存在多个形态和功能上可辨别的IPL分层。值得注意的是，即使在可检测到典型IPL标记物变化之前，糖尿病大鼠模型中IPL的分层模式就已严重破坏。这些发现表明，TNAP组织化学为研究视网膜分层及其糖尿病诱导的退化提供了一种更直接、但也更敏感的方法。
