用铈法在经微波炉处理的肾脏树脂切片中对磷酸酶活性进行光镜观察。
Light microscopical visualization of phosphatase activities demonstrated with the cerium method in resin sections of the kidney with the microwave oven.
作者信息
Kalicharan D, Hulstaert C E
机构信息
Centre for Medical Electron Microscopy, University of Groningen, The Netherlands.
出版信息
Histochemistry. 1989;90(6):485-7. doi: 10.1007/BF00494360.
The light microscopically invisible reaction product cerium phosphate in resin sections of rat kidney, that had been incubated for the demonstration of phosphatase activities before embedding, was converted into a visible reaction product by incubation for 10 min at 80 degrees C in alkaline lead citrate in a microwave oven. This method offers the possibility to study phosphatase activities with the cerium method in semithin Epon sections. Furthermore it is a suitable method to select areas with phosphatase activity to be studied with the electron microscope.
大鼠肾脏树脂切片中在包埋前为显示磷酸酶活性而孵育的光镜下不可见的反应产物磷酸铈，通过在微波炉中于碱性柠檬酸铅中80℃孵育10分钟转化为可见反应产物。该方法为在半薄环氧树脂切片中用铈法研究磷酸酶活性提供了可能。此外，它是一种选择具有磷酸酶活性区域以供电子显微镜研究的合适方法。
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