Angermüller S, Fahimi H
Histochemistry. 1984;80(2):107-11. doi: 10.1007/BF00679983.
The use of cerium chloride for the localization of thiamine-pyrophosphatase (TPPase) in rat liver parenchymal cells has been investigated and the results are compared with the classical lead capture method. A medium containing 3 mM cerium chloride gave the most uniform and consistent results with a homogeneous electron dense reaction product in the first trans lamella of the Golgi complex and a weak staining of endoplasmic reticulum. The fine deposits of cerium phosphate filled completely the first trans Golgi cisterna. In contrast the reaction product of the lead-based method appeared clumpy and aggregated with an irregular distribution over both Golgi complex and endoplasmic reticulum. Higher and lower concentrations of cerium chloride than 3 mM gave inconsistent results. The present study demonstrates that the cerium-based method is superior to the classical lead-technique for the localization of TPPase.
研究了氯化铈用于大鼠肝实质细胞中硫胺素焦磷酸酶（TPPase）定位的情况，并将结果与经典的铅捕获法进行了比较。含有3 mM氯化铈的培养基产生了最均匀一致的结果，在高尔基复合体的第一个反式扁平囊中有均匀的电子致密反应产物，内质网有微弱染色。磷酸铈的细微沉积物完全填满了高尔基复合体的第一个反式扁平囊。相比之下，基于铅的方法的反应产物显得结块且聚集，在高尔基复合体和内质网中分布不规则。高于和低于3 mM的氯化铈浓度产生的结果不一致。本研究表明，基于铈的方法在TPPase定位方面优于经典的铅技术。
