基于苯乙烯基菁染料的pH荧光探针的活细胞荧光成像及细胞毒性评估
[Live cell fluorescent imaging and cytotoxicity assessment of pH fluorescent probe based on styrylcyanine dyes].
作者信息
Huang Lixia, Liu Xiang, Cui Danting, Xu Yikai, You Wenwei, Yan Yichen, Lu Xinwei, Liu Ruiyuan
机构信息
Medical Imaging Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Nov;34(11):1642-5.
OBJECTIVE
To prepare a pH fluorescence probe based on styrylcyanine dyes for live cell imaging.
METHODS
The Probe 1 was prepared by reaction of 4-pyridinecarboxaldehyde with 1,1,2-trimethylbenz[e]indole. The influence of pH on the fluorescent properties was examined, and the cell viability was examined using cell counting kit-8. The Probe 1 was used as a pH fluorescence probe in living cell.
RESULTS
Probe 1 emitted green fluorescence under neutral and basic conditions but orange fluorescence under acid condition. Probe 1 selectively stained the cytoplasmic regions of living cells without significantly affecting the cell viability.
CONCLUSION
The pH-sensitive fluorescent probe prepared based on styrylcyanine possesses good ability of cell membrane permeation for live cell fluorescent imaging.
目的
制备一种基于苯乙烯基菁染料的pH荧光探针用于活细胞成像。
方法
通过4-吡啶甲醛与1,1,2-三甲基苯并[e]吲哚反应制备探针1。考察了pH对荧光性质的影响，并使用细胞计数试剂盒-8检测细胞活力。将探针1用作活细胞中的pH荧光探针。
结果
探针1在中性和碱性条件下发出绿色荧光，而在酸性条件下发出橙色荧光。探针1选择性地对活细胞的细胞质区域进行染色，而对细胞活力没有显著影响。
结论
基于苯乙烯基菁制备的pH敏感荧光探针具有良好的细胞膜渗透能力，可用于活细胞荧光成像。
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