Department of Chemistry, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476 Potsdam (Germany); School of Analytical Sciences Adlershof, Humboldt-Universität zu Berlin, Unter den Linden 6, 10099 Berlin (Germany).
Angew Chem Int Ed Engl. 2015 Jan 7;54(2):673-7. doi: 10.1002/anie.201409278. Epub 2014 Nov 20.
DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na(+) and K(+)) is currently used for the detection of K(+) ions, however, with insufficient selectivity towards Na(+). By means of FRET between two suitable dyes attached to the 3'- and 5'-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.
DNA 折纸纳米结构是一种多功能工具，可用于以高局部控制来排列功能，以在单分子水平上研究分子过程。在这里，我们证明 DNA 折纸基底可用于抑制端粒 DNA 形成特定的鸟嘌呤 (G) 四链体结构。在单价阳离子（例如 Na(+) 和 K(+)）存在下，端粒折叠成 G-四链体结构目前用于检测 K(+) 离子，但是对 Na(+) 的选择性不足。通过附着在端粒 DNA 的 3' 和 5' 末端的两个合适染料之间的 FRET，我们证明由于空间位阻，在钠离子存在下，在 DNA 折纸模板上形成 G-四链体的过程受到抑制。因此，附着在 DNA 折纸结构上的端粒 DNA 代表了一种对钾离子具有高灵敏度和选择性的检测工具，即使在存在高浓度钠离子的情况下也是如此。
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