产头孢菌素酸合成酶的大肠杆菌VKPM B-10182菌株的基因组序列草图

Draft Genome Sequence of Escherichia coli Strain VKPM B-10182, Producing the Enzyme for Synthesis of Cephalosporin Acids.

作者信息

Mardanov Andrey V, Eldarov Mikhail A, Sklyarenko Anna V, Dumina Maria V, Beletsky Alexey V, Yarotsky Sergey V, Ravin Nikolai V

机构信息

Centre "Bioengineering," Russian Academy of Sciences, Moscow, Russian Federation.

State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.

出版信息

Genome Announc. 2014 Nov 20;2(6):e01222-14. doi: 10.1128/genomeA.01222-14.

DOI:10.1128/genomeA.01222-14

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4239367/

Abstract

Escherichia coli strain VKPM B-10182, obtained by chemical mutagenesis from E. coli strain ATCC 9637, produces cephalosporin acid synthetase employed in the synthesis of β-lactam antibiotics, such as cefazolin. The draft genome sequence of strain VKPM B-10182 revealed 32 indels and 1,780 point mutations that might account for the improvement in antibiotic synthesis that we observed.

摘要

大肠杆菌菌株VKPM B-10182是通过对大肠杆菌菌株ATCC 9637进行化学诱变获得的，它能产生用于合成β-内酰胺抗生素（如头孢唑林）的头孢菌素酸合成酶。菌株VKPM B-10182的基因组草图序列显示有32个插入缺失和1780个点突变，这些可能是我们观察到的抗生素合成改善的原因。

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