被史密斯甲烷短杆菌古脂质体包裹的乙肝表面抗原诱导强烈且特异性的体液免疫和辅助性T细胞1型细胞免疫反应
Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes.
作者信息
Aghasadeghi Mohammad Reza, Delbaz Seyed Ali, Sadat Seyed Mehdi, Siadat Seyed Davar, Ardestani Mehdi Shafiee, Rahimi Pooneh, Bolhassani Azam, Roudsari Rouhollah Vahabpour, Bahramali Golnaz, Motevalli Fateme, Davari Mehdi, Vakily Habib, Salmani Ali Sharifat, Nobari Maryam Borhan
机构信息
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
出版信息
Avicenna J Med Biotechnol. 2014 Oct;6(4):238-45.
BACKGROUND
Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features.
METHODS
In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed.
RESULTS
Frequency of IFN-γ secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg(+)C/IFA groups (p≤0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤ 0.05).
CONCLUSION
Analysis of induced responses revealed the immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-γ secreting splenocytes of immunized mice.
背景
应用源自微生物的佐剂是疫苗学试验中最近备受关注的一种方法。古脂质体是具有佐剂和脂质体活性及特征的微生物化合物之一。
方法
在本研究中，将重组乙肝表面抗原（HBsAg）包封于史氏甲烷短杆菌（M. smithii）古脂质体中。用该化合物免疫Balb/c小鼠，并分析免疫模型的体液和细胞因子分泌模式。
结果
含HBsAg的古脂质体组中分泌干扰素-γ（IFN-γ）的细胞频率显著高于HBsAg组和HBsAg(+)C/IFA组（p≤0.05）。含HBsAg的古脂质体组血清中的IgG2a滴度也显著高于其他组中该亚类的滴度（p≤0.05）。
结论
诱导反应分析显示，根据免疫小鼠中IgG2a亚型和分泌IFN-γ的脾细胞占优势，史氏甲烷短杆菌古脂质体在诱导辅助性T细胞1反应方面具有免疫增强特性。
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