马铃薯（茄属马铃薯种）。

Potato (Solanum tuberosum L.).

作者信息

Chetty Venkateswari J, Narváez-Vásquez Javier, Orozco-Cárdenas Martha L

机构信息

Plant Transformation Research Center, University of California Riverside, South Campus Dr. Head House No.2, Riverside, CA, 92507-0124, USA.

出版信息

Methods Mol Biol. 2015;1224:85-96. doi: 10.1007/978-1-4939-1658-0_8.

DOI:10.1007/978-1-4939-1658-0_8

PMID:25416251

Abstract

Agrobacterium-mediated transformation is the most common method for the incorporation of foreign genes into the genome of potato as well as many other species in the Solanaceae family. This chapter describes protocols for the genetic transformation of three species of potato: Solanum tuberosum subsp. tuberosum (Desiréé), S. tuberosum subsp. andigenum (Blue potato), and S. tuberosum subsp. andigena using internodal segments as explants.

摘要

农杆菌介导的转化是将外源基因导入马铃薯基因组以及茄科许多其他物种基因组的最常用方法。本章描述了三种马铃薯品种的遗传转化方案：普通栽培种马铃薯（德西蕾）、智利马铃薯亚种（蓝马铃薯）和智利马铃薯亚种，以外植体节间段为材料。