马铃薯的质体转化：营养和工业材料的重要来源。

Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials.

机构信息

CNR-IBBR, National Research Council of Italy, Institute of Biosciences and BioResources, Research Division Portici, Portici (NA), Italy.

CREA-OF, Research Centre for Vegetable and Ornamental Crops, Pontecagnano (SA), Italy.

出版信息

Methods Mol Biol. 2021;2317:247-256. doi: 10.1007/978-1-0716-1472-3_13.

DOI:10.1007/978-1-0716-1472-3_13

PMID:34028773

Abstract

For a long time, plastid transformation has been a routine technology only in tobacco due to lack of effective selection and regeneration protocols, and, for some species, due to inefficient recombination using heterologous flanking regions in transformation vectors. Nevertheless, the availability of this technology to economically important crops offers new possibilities in plant breeding to manage pathogen resistance or improve nutritional value. Herein we describe an efficient plastid transformation protocol for potato (Solanum tuberosum subsp. tuberosum), achieved by the optimization of the tissue culture procedures and using transformation vectors carrying homologous potato flanking sequences. This protocol allowed to obtain up to one shoot per shot, an efficiency comparable to that usually accomplished in tobacco. Further, the method described in this chapter has been successfully used to regenerate potato transplastomic plants expressing recombinant GFP protein in chloroplasts and amyloplasts or long double-stranded RNAs for insect pest control.

摘要

长期以来，由于缺乏有效的选择和再生方案，质体转化一直是烟草中的常规技术，对于某些物种，由于转化载体中外源侧翼区域的重组效率低下，也无法实现质体转化。然而，这项技术在经济上重要的作物中的应用为植物育种提供了新的可能性，可以管理病原体抗性或提高营养价值。本文描述了一种高效的马铃薯（Solanum tuberosum subsp. tuberosum）质体转化方案，通过优化组织培养程序和使用携带同源马铃薯侧翼序列的转化载体来实现。该方案可达到每外植体 1 个芽的效率，与烟草中通常达到的效率相当。此外，本章中描述的方法已成功用于再生表达叶绿体和淀粉体中重组 GFP 蛋白或用于防治昆虫的长双链 RNA 的马铃薯质体转化植株。