来自尼泊尔西部的八种药用植物的抗氧化活性及酚类和类黄酮含量。

Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal.

出版信息

J Tradit Chin Med. 2014 Oct;34(5):584-90. doi: 10.1016/s0254-6272(15)30067-4.

Abstract

OBJECTIVE

The purpose of this study was to evaluate the antioxidant activity, phenolic and flavonoids contents of selected medicinal plants form the Western region of Nepal.

METHODS

The antioxidant activity of selected medicinal plants were determined by using 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity; total ferric ion reducing ability through spectroscopic analysis. The content of total phenols was determined using Folin-Ciocalteu reagent, whereas Aluminum chloride colorimetric method was used for flavonoid determination.

RESULTS

Extracts of Syzygium Operculatus (87%), Astilbe Rivularis (83%) and Mallotus Philippnensis (88%) showed significant free radical scavenging activity with effective concentration (EC50) close to that of ascorbic acid. Syzygium Operculatus (96%), Astilbe Rivularis (97%) and Mallotus Philippnensis(97%) had potent reducing power in concentration dependent fashion. Those plant extract with higher free radical scavenging and ferric reducing effect also showed the greater content of both phenols and flavonoids, suggesting the correlation between polyphenolic content and antioxidant activity. Those plant extracts which showed better antioxidant activity assays, also demonstrated higher total phenol and flavonoid contents. These three plants showed the presence of higher amount of phenols and flavonoids.

CONCLUSION

This study may provide the scientific basis for the traditional use of those plants and may provide valuable idea for further research.

摘要

目的

本研究旨在评估来自尼泊尔西部地区的几种药用植物的抗氧化活性、酚类和类黄酮含量。

方法

采用 2,2-二苯基-1-苦基肼自由基清除活性法和光谱分析测定法测定所选药用植物的抗氧化活性。采用福林-考卡尔特试剂测定总酚含量，采用铝氯化物比色法测定类黄酮含量。

结果

Syzygium Operculatus（87%）、Astilbe Rivularis（83%）和 Mallotus Philippnensis（88%）的提取物表现出显著的自由基清除活性，其有效浓度（EC50）接近抗坏血酸。Syzygium Operculatus（96%）、Astilbe Rivularis（97%）和 Mallotus Philippnensis（97%）具有浓度依赖性的强还原能力。那些具有较高自由基清除和铁还原效果的植物提取物也表现出较高的酚类和类黄酮含量，表明多酚含量与抗氧化活性之间存在相关性。在抗氧化活性测定中表现较好的植物提取物也显示出较高的总酚和类黄酮含量。这三种植物均表现出较高的酚类和类黄酮含量。