Saieg Amarel, Brown Kristy J, Pena Maria T, Rose Mary C, Preciado Diego
Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC.
1] Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC [2] Department of Integrative Systems Biology, Children's National Medical Center, Washington, DC.
Pediatr Res. 2015 Feb;77(2):356-62. doi: 10.1038/pr.2014.187. Epub 2014 Nov 24.
Chronic rhinosinusitis (CRS) is characterized by mucous overproduction and submucosal gland hyperplasia. The global protein profile of sinonasal secretions in pediatric CRS has not been studied. We hypothesized that MUC5B, a glandular mucin, would be relatively increased in CRS secretions compared to other mucins.
Secretions were collected at Children's National Health System (Children's National) from CRS patients undergoing sinus surgery and from control patients without CRS undergoing craniofacial procedures. Proteins were extracted, digested to peptides, and analyzed by mass spectometry. Fold change significance was calculated using the QSpec algorithm. Western blot analysis was performed to validate proteomic findings.
In total, 294 proteins were identified. Although both MUC5B and MUC5AC were identified in a majority of samples, the relative abundance of MUC5B was found to be significantly higher (P < 0.05). Western blot data validated these findings. Other proteins with the highest significant positive-fold change in CRS samples were BP1 fold-containing family A member 1, chitinase-3-like protein 1, plastin-2, serpin 10, and BP1 fold-containing family B member 1.
Overall, our data demonstrate an increase of MUC5B abundance in the sinus secretions of pediatric patients with CRS.
慢性鼻-鼻窦炎（CRS）的特征是黏液过度分泌和黏膜下腺体增生。小儿CRS鼻窦分泌物的整体蛋白质谱尚未得到研究。我们推测，与其他黏蛋白相比，腺性黏蛋白MUC5B在CRS分泌物中会相对增加。
在儿童国家卫生系统（儿童国家）收集接受鼻窦手术的CRS患者以及接受颅面手术的无CRS对照患者的分泌物。提取蛋白质，消化成肽，并通过质谱分析。使用QSpec算法计算倍数变化的显著性。进行蛋白质印迹分析以验证蛋白质组学结果。
总共鉴定出294种蛋白质。虽然大多数样品中都鉴定出了MUC5B和MUC5AC，但发现MUC5B的相对丰度显著更高（P < 0.05）。蛋白质印迹数据验证了这些结果。CRS样品中具有最高显著正倍数变化的其他蛋白质是含BP1折叠家族A成员1、几丁质酶-3-样蛋白1、质体肌动蛋白-2、丝氨酸蛋白酶抑制剂10和含BP1折叠家族B成员1。
总体而言，我们的数据表明小儿CRS患者鼻窦分泌物中MUC5B丰度增加。
