Ma Zhi-Bin, Kong Xiao-Lin, Cui Gang, Ren Cui-Cui, Zhang Ying-Jie, Fan Sheng-Jin, Li Ying-Hua
Department of Gastroenterology, the First Affiliated Hospital of Harbin Medical University, Harbin, China E-mail :
Asian Pac J Cancer Prev. 2014;15(21):9265-70. doi: 10.7314/apjcp.2014.15.21.9265.
The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions.
Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA- 34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a.
After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05).
miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.
本研究旨在调查miRNA - 34a在良性和恶性结直肠病变中的表达差异。
选取癌症、癌旁组织和息肉样本，采用常规方法提取总RNA，进行实时荧光定量PCR以检测miRNA - 34a的表达。此外，培养LOVO结直肠癌细胞系，用去甲基化剂5 - 氮杂胞苷处理，并筛选差异表达的miRNA - 34a。
药物处理后，结直肠癌细胞系LOVO的miRNA - 34a表达增加，实时荧光定量PCR显示，与癌旁组织相比，细胞系和结直肠癌组织中的表达水平均较低（p<0.05）。息肉组织的表达明显高于癌旁组织和结直肠癌样本（p<0.05）。
miRNA - 34a - 5p可能作为一种肿瘤抑制基因在结直肠癌中发挥作用，且与DNA甲基化有关。
