血浆基质金属蛋白酶-11水平升高与口腔鳞状细胞癌患者的临床病理特征相关。
High level of plasma matrix metalloproteinase-11 is associated with clinicopathological characteristics in patients with oral squamous cell carcinoma.
作者信息
Hsin Chung-Han, Chen Mu-Kuan, Tang Chih-Hsin, Lin Huang-Pin, Chou Ming-Yung, Lin Chiao-Wen, Yang Shun-Fa
机构信息
School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.
出版信息
PLoS One. 2014 Nov 25;9(11):e113129. doi: 10.1371/journal.pone.0113129. eCollection 2014.
BACKGROUND
Matrix metalloproteinase-11 (MMP-11) is reported to be overexpressed in several cancers and may contribute to tumorigenesis. The current study investigated the association between the clinicopathological characteristics and plasma level of MMP-11 in oral squamous cell carcinoma (OSCC) patients.
METHODOLOGY AND PRINCIPAL FINDINGS
The plasma MMP-11 concentration was determined by ELISA on 330 male OSCC patients. In addition, the metastatic effects of the MMP-11 knockdown on the oral cancer cells were investigated by cell migration assay. Our results showed that the plasma MMP-11 levels were significantly higher in patients with advanced T status (p = 0.001), lymph node metastasis (p = 0.006) and higher TNM stages (p<0.001). Moreover, treatment with the MMP-11 shRNA exerted an inhibitory effect on migration in SCC9 oral cancer cells.
CONCLUSION
Our study showed that plasma level of MMP-11 may be useful for assessment of the disease progression, especially lymph node metastasis, in patients with OSCC.
背景
据报道，基质金属蛋白酶-11（MMP-11）在多种癌症中过度表达，可能与肿瘤发生有关。本研究调查了口腔鳞状细胞癌（OSCC）患者的临床病理特征与血浆MMP-11水平之间的关联。
方法和主要发现
采用酶联免疫吸附测定（ELISA）法测定330例男性OSCC患者血浆中的MMP-11浓度。此外，通过细胞迁移试验研究了MMP-11基因敲低对口腔癌细胞转移的影响。我们的结果显示，T分期较晚（p = 0.001）、有淋巴结转移（p = 0.006）和TNM分期较高（p<0.001）的患者血浆MMP-11水平显著更高。此外，用MMP-11短发夹RNA（shRNA）处理对SCC9口腔癌细胞的迁移有抑制作用。
结论
我们的研究表明，血浆MMP-11水平可能有助于评估OSCC患者的疾病进展，尤其是淋巴结转移情况。
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