基质金属蛋白酶-7（MMP-7）和膜型基质金属蛋白酶-1（MT1-MMP）在口腔鳞状细胞癌中的表达作为肿瘤侵袭和预后的预测指标
Expression of MMP-7 and MT1-MMP in oral squamous cell carcinoma as predictive indicator for tumor invasion and prognosis.
作者信息
de Vicente J-C, Lequerica-Fernández P, Santamaría J, Fresno M-F
机构信息
Servicio de Cirugía Maxilofacial, Hospital Universitario Central de Asturias, Facultad de Medicina y Odontología, Instituto Universitario de Oncología del Principado de Asturias, Oviedo, Spain.
出版信息
J Oral Pathol Med. 2007 Aug;36(7):415-24. doi: 10.1111/j.1600-0714.2007.00546.x.
BACKGROUND
Squamous cell carcinoma of the oral cavity is a highly invasive neoplasm that spreads locally and metastasizes to regional lymph nodes. This process involves multiple proteolytic enzymes including matrilysin (MMP-7) and membrane type I-matrix metalloproteinase (MT1-MMP). This study was designed to explore the association between MMP-7 and MT1-MMP in the invasiveness and prognosis of oral squamous cell carcinoma (OSCC).
METHODS
About 4-microM, formalin-fixed, paraffin-embedded tissue sections from 69 patients with OSCC were immunohistochemically studied using specific antibodies against MMP-7 and MT1-MMP proteins. Immunostaining was semiquantitatively scored, and results were correlated with histologic and clinical variables including clinical behavior and survival.
RESULTS
MMP-7 was observed only in cancer cells, and MT1-MMP in both tumoral tissue and stroma. MMP-7 expression was significantly correlated with lymph node metastasis (P = 0.03; RR = 3.2). MT1-MMP showed a significant association with TIMP-2 (in N+ cases) and p53 expression (P = 0.01). MMP-7 and MT1-MMP displayed a survival relevance, and in multivariate analysis they were independent prognostic indicators, particularly in neck node-positive cases.
背景
口腔鳞状细胞癌是一种具有高度侵袭性的肿瘤，可在局部扩散并转移至区域淋巴结。这一过程涉及多种蛋白水解酶，包括基质溶素（MMP-7）和膜型I基质金属蛋白酶（MT1-MMP）。本研究旨在探讨MMP-7和MT1-MMP在口腔鳞状细胞癌（OSCC）侵袭性及预后中的关联。
方法
采用抗MMP-7和MT1-MMP蛋白的特异性抗体，对69例OSCC患者经4微米福尔马林固定、石蜡包埋的组织切片进行免疫组织化学研究。对免疫染色进行半定量评分，并将结果与包括临床行为和生存情况在内的组织学及临床变量相关联。
结果
MMP-7仅在癌细胞中观察到，而MT1-MMP在肿瘤组织和基质中均有表达。MMP-7表达与淋巴结转移显著相关（P = 0.03；相对危险度 = 3.2）。MT1-MMP与TIMP-2（在N+病例中）及p53表达显著相关（P = 0.01）。MMP-7和MT1-MMP显示出与生存的相关性，在多因素分析中它们是独立的预后指标，尤其是在颈部淋巴结阳性病例中。
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