稳定型冠状动脉疾病患者红细胞中膜脂质过氧化增加及钠钾ATP酶活性降低。
Increased membrane lipid peroxidation and decreased Na+/K+-ATPase activity in erythrocytes of patients with stable coronary artery disease.
作者信息
Namazi Gholamreza, Jamshidi Rad Sabieh, Attar Ahmad Movahedian, Sarrafzadegan Nizal, Sadeghi Masoumeh, Naderi Gholamali, Pourfarzam Morteza
机构信息
aDepartment of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences bIsfahan Cardiovascular Research Center cCardiac Rehabilitation Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Coron Artery Dis. 2015 May;26(3):239-44. doi: 10.1097/MCA.0000000000000196.
OBJECTIVES
We aimed to determine erythrocyte membrane lipid peroxidation levels and Na/K-ATPase activity in patients with and without coronary artery disease (CAD) documented by coronary angiography.
DESIGN AND METHODS
A total of 144 patients who had undergone coronary angiography were divided into a CAD group (n=82) and a non-CAD group (control group, n=62) according to the results of coronary angiography. Lipid peroxide levels in plasma and the erythrocyte membrane were measured using a fluorimetric method. Total antioxidant status and Na/K-ATPase activity in plasma were determined using spectrophotometric methods.
RESULTS
Lipid peroxidation levels were significantly higher in the erythrocyte membrane of CAD patients compared with controls, whereas Na/K-ATPase activity was significantly lower in the erythrocyte membrane of CAD patients compared with controls. The coronary artery scores were correlated positively with membrane lipid peroxidation (r=0.324, P<0.001) and negatively with Na/K-ATPase activity (r=-0.302, P<0.001).
CONCLUSION
This study shows that the levels of membrane lipid peroxidation and Na/K-ATPase activity are correlated with the severity of CAD.
目的
我们旨在确定经冠状动脉造影证实患有和未患有冠状动脉疾病（CAD）的患者的红细胞膜脂质过氧化水平和钠钾ATP酶活性。
设计与方法
根据冠状动脉造影结果，将总共144例行冠状动脉造影的患者分为CAD组（n = 82）和非CAD组（对照组，n = 62）。采用荧光法测量血浆和红细胞膜中的脂质过氧化物水平。采用分光光度法测定血浆中的总抗氧化状态和钠钾ATP酶活性。
结果
与对照组相比，CAD患者红细胞膜中的脂质过氧化水平显著更高，而CAD患者红细胞膜中的钠钾ATP酶活性显著更低。冠状动脉评分与膜脂质过氧化呈正相关（r = 0.324，P < 0.001），与钠钾ATP酶活性呈负相关（r = -0.302，P < 0.001）。
结论
本研究表明，膜脂质过氧化水平和钠钾ATP酶活性与CAD的严重程度相关。
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