Mohammadi Amir Hossein, Balandeh Ebrahim, Hasani Jila, Karimian Mohammad, Arabshahi Vajiheh, Pourfarzam Morteza, Bahmani Fereshteh, Namazi Gholamreza
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran.
Student Research Committee, Kashan University of Medical Sciences, Kashan, Iran.
Biomed Res Int. 2024 Feb 23;2024:9979582. doi: 10.1155/2024/9979582. eCollection 2024.
Oxidative stress is involved in pathogenesis of some psychiatric disorders. To examine the role of oxidative stress in the etiopathogenesis of obsessive-compulsive disorder (OCD), we aimed to determine oxidative stress indices, including malondialdehyde (MDA) levels in serum and red blood cells (RBC) membrane, total antioxidant capacity (TAC), serum glutathione (GSH) levels, serum antioxidant vitamins (A and E), and Na/K-ATPase activity, in patients with the mentioned disorder vs. healthy controls.
39 OCD patients diagnosed based on Diagnostic and Statistical Manual of Mental Disorders (DSM-V) and 39 volunteer healthy subjects were included in this study. MDA levels in serum and RBC membrane were measured using fluorometric method. Serum TAC level, serum GSH level, and Na/K-ATPase activity were also measured using spectrophotometric methods. Serum levels of vitamins were calculated by reversed-phase high-performance liquid chromatography (RP-HPLC).
There was a significantly higher MDA level in serum ( < 0.0001) and RBC membrane ( = 0.002) of OCD patients compared with those in controls. A significant reduction in vitamin A ( = 0.001) and vitamin E ( = 0.024) levels was found in OCD patients vs. controls. There was significantly lower activity of erythrocyte membrane Na/K-ATPase in RBC membrane of OCD patients vs. controls ( < 0.0001).
Our findings indicate significantly higher levels MDA in both serum and RBC membrane, lower levels of serum vitamins A and E, and lower activity of membrane Na/K-ATPase in OCD patients compared to controls. These suggest an imbalance between oxidant and antioxidant factors in OCD patients that might play a fundamental role in the etiopathogenesis of OCD.
氧化应激参与了某些精神障碍的发病机制。为了研究氧化应激在强迫症（OCD）病因学中的作用，我们旨在确定氧化应激指标，包括血清和红细胞（RBC）膜中的丙二醛（MDA）水平、总抗氧化能力（TAC）、血清谷胱甘肽（GSH）水平、血清抗氧化维生素（A和E）以及Na/K - ATP酶活性，以比较上述疾病患者与健康对照者。
本研究纳入了39例根据《精神障碍诊断与统计手册》（DSM - V）诊断的强迫症患者和39名志愿者健康受试者。采用荧光法测量血清和RBC膜中的MDA水平。还采用分光光度法测量血清TAC水平、血清GSH水平和Na/K - ATP酶活性。通过反相高效液相色谱法（RP - HPLC）计算血清维生素水平。
与对照组相比，强迫症患者血清（<0.0001）和RBC膜（=0.002）中的MDA水平显著更高。与对照组相比，强迫症患者的维生素A（=0.001）和维生素E（=0.024）水平显著降低。与对照组相比，强迫症患者RBC膜中红细胞膜Na/K - ATP酶的活性显著降低（<0.0001）。
我们的研究结果表明，与对照组相比，强迫症患者血清和RBC膜中的MDA水平显著更高，血清维生素A和E水平更低，膜Na/K - ATP酶活性更低。这些表明强迫症患者体内氧化和抗氧化因子之间存在失衡，这可能在强迫症的病因学中起重要作用。
