Kirsch David, Lowen Marcia Serva, Fialho Cronemberger Mônica Fialho, Sato Elcio Hideo
J Pediatr Ophthalmol Strabismus. 2014 Nov-Dec;51(6):341-7. doi: 10.3928/01913913-20141021-06.
To evaluate the effect of amniotic membrane in reducing inflammation, fibrosis, adhesion formation, and ocular motility restrictions following strabismus surgery.
In the first stage of this prospective, randomized study, 17 rabbits underwent superior rectus muscle recession in both eyes. Surgery was performed in the same manner, but human amniotic membrane was placed over the muscle without sutures in the right eye after recession. After 15 days, the rabbits were killed and their orbits were exenterated and evaluated histopathologically to quantify tissue inflammation and fibrosis. In the second stage, 5 rabbits underwent the same procedure but were killed after 30 days. A dynamometer was used to measure the force required to displace all eyes.
At 15 days postoperatively, eyes with amniotic membrane exhibited an increased inflammatory response and less fibrosis than eyes without amniotic membrane. At 30 days postoperatively, eyes with amniotic membrane continued to exhibit increased inflammation and less fibrosis than eyes without amniotic membrane. In the dynamometer test, more force was needed to displace eyes without amniotic membrane after 15 days, but there was no significant difference between the forces required at 30 days.
Human amniotic membrane in rabbits led to an increase in the inflammatory process and a decrease in fibrosis formation following strabismus surgery.
评估羊膜在减少斜视手术后炎症、纤维化、粘连形成及眼球运动受限方面的作用。
在这项前瞻性随机研究的第一阶段，17只兔子双眼均行上直肌后徙术。手术方式相同，但右眼后徙术后将人羊膜置于肌肉表面且不缝合。15天后，处死兔子并摘除眼眶，进行组织病理学评估以量化组织炎症和纤维化程度。在第二阶段，5只兔子接受相同手术操作，但在30天后处死。使用测力计测量移动所有眼球所需的力量。
术后15天，有羊膜的眼比无羊膜的眼炎症反应增强且纤维化程度减轻。术后30天，有羊膜的眼与无羊膜的眼相比，炎症仍增强且纤维化程度仍减轻。在测力计测试中，术后15天移动无羊膜的眼需要更大的力量，但30天时所需力量无显著差异。
兔子使用人羊膜导致斜视手术后炎症过程增加，纤维化形成减少。
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