Ophthalmology Department, Cairo University, Egypt.
Curr Eye Res. 2011 Nov;36(11):1020-7. doi: 10.3109/02713683.2011.601842. Epub 2011 Sep 23.
To histopathologically evaluate the influence of lyophilized human amniotic membrane transplant on the development of postoperative adhesions and fibrosis after extraocular muscle surgery.
Ten albino rabbits were used. The superior rectus muscle was resected 4 mm in both eyes. In right eyes, the superior rectus muscle was wrapped with lyophilized (air-dried, freeze-dried) human amniotic membrane (group AM). In left eyes, the superior rectus muscle was not wrapped with amniotic membrane, and served as a control group (group C). The rabbits were sacrificed and the eyes were enucleated 6 weeks after surgery to perform histopathological examination.
Two rabbits died 1 week after surgery and were excluded. Histopathological evaluation of both eyes of the remaining 8 rabbits was done. The amniotic membrane was not seen in all eyes. On comparing eye pairs of each rabbit, right eyes showed significantly less conjunctival inflammation (p = 0.034), but insignificantly less foreign body inflammation (p = 0.625), adhesions between sclera and muscle (p = 0.206), muscle fibrosis (p = 1.000), and conjunctival hyperemia (p = 0.059) as compared to left eyes.
Lyophilized amniotic membrane insignificantly reduces postoperative adhesions and fibrosis, when used to wrap the operated upon extraocular muscles, limiting the benefit of this membrane in strabismus surgery.
通过组织病理学评估，研究冻干人羊膜移植对外直肌手术后粘连和纤维化形成的影响。
使用 10 只白化兔。双眼均切除 4mm 外直肌。右眼外直肌包裹冻干（风干、冷冻干燥）人羊膜（AM 组），左眼不包裹羊膜作为对照组（C 组）。术后 6 周处死兔子并眼球摘出，进行组织病理学检查。
术后 1 周有 2 只兔子死亡，被排除。对其余 8 只兔子的双眼进行组织病理学评估。所有眼睛均未见羊膜。比较每组兔子的双眼，右眼的结膜炎症明显较轻（p=0.034），但异物炎症无明显差异（p=0.625），巩膜和肌肉之间的粘连（p=0.206）、肌肉纤维化（p=1.000）和结膜充血（p=0.059）较轻。
冻干羊膜包裹术中外直肌，术后粘连和纤维化程度轻微减轻，但对外斜视手术的益处有限。
