Chung Tae-Ho, Choi Hee Soo, Lee Choong Hyun
Department of Animal Resources Science, Joongbu University, Chungnam 312-702, South Korea.
J Vet Med Sci. 2015 Mar;77(3):349-52. doi: 10.1292/jvms.14-0509. Epub 2014 Nov 26.
A previous study showed that 1 mg/kg lipopolysaccharide (LPS) treatment did not lead to the any neuronal death/degeneration in the mouse hippocampus. In the present study, we examined the time-dependent changes of calbindin D-28k (CB) protein expression in the mouse hippocampus after a systemic administration of 1 mg/kg LPS. CB immunoreactivity was markedly increased in pyramidal cells of the hippocampal CA1/2 regions and in granule cells of the dentate gyrus from 3 hr to 48 hr after LPS treatment. At this point in time, CB protein level was also significantly increased in the mouse hippocampus. Thereafter, CB protein expression was decreased at 96 hr after LPS treatment. These results indicate that changes of CB protein expression may be associated with no neuronal death in the model of neuroinflammation with systemic administration of 1 mg/kg LPS.
先前的一项研究表明，1mg/kg脂多糖（LPS）处理并未导致小鼠海马体出现任何神经元死亡/退化。在本研究中，我们检测了全身给予1mg/kg LPS后小鼠海马体中钙结合蛋白D-28k（CB）蛋白表达的时间依赖性变化。LPS处理后3小时至48小时，海马CA1/2区锥体细胞和齿状回颗粒细胞中的CB免疫反应性显著增加。此时，小鼠海马体中的CB蛋白水平也显著升高。此后，LPS处理96小时后CB蛋白表达下降。这些结果表明，在全身给予1mg/kg LPS的神经炎症模型中，CB蛋白表达的变化可能与无神经元死亡有关。
