Fournier F, Honoré E, Brûlé G, Mironneau J, Guilbault P
Laboratoire de Physiologie Cellulaire, Université de Lille I, villeneuve d'Ascq, France.
Pflugers Arch. 1989 Apr;413(6):682-4. doi: 10.1007/BF00581822.
Using the single electrode voltage-clamp technique, stage V and VI Xenopus oocytes showed a Ba inward current (endogenous IBa) with a peak amplitude of -15 +/- 2 nA in a Cl and Na-free Ba methane sulphonate medium. When oocytes were injected with pregnant rat (18 days gestation) myometrium mRNA, an additional component of Ba current could be detected (exogenous IBa). This inward current could be distinguished from the native IBa by several means: i) peak amplitude (-75 +/- 3 nA); ii) activation voltage threshold; iii) steady-state inactivation parameter; iv) sensitivity to dihydropyridines. The features of the exogenous IBa were compared to those of the inward Ca channel current recorded with the tight-seal patch-clamp technique in single myometrial cells maintained in primary culture.
采用单电极电压钳技术，在无氯和无钠的甲磺酸钡培养基中，Ⅴ期和Ⅵ期非洲爪蟾卵母细胞表现出内向钡电流（内源性 IBa），其峰值幅度为 -15±2 nA。当向卵母细胞注射妊娠大鼠（妊娠 18 天）子宫肌层 mRNA 时，可检测到额外的钡电流成分（外源性 IBa）。这种内向电流可通过多种方式与天然 IBa 区分开来：i）峰值幅度（-75±3 nA）；ii）激活电压阈值；iii）稳态失活参数；iv）对二氢吡啶的敏感性。将外源性 IBa 的特征与在原代培养的单个子宫肌层细胞中用紧密封膜片钳技术记录的内向钙通道电流的特征进行了比较。
