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猴病毒40（SV40）大T抗原在DNA复制起始过程中会引起SV40起始结构的逐步变化。

Simian virus 40 (SV40) large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication.

作者信息

Roberts J M

机构信息

Department of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

出版信息

Proc Natl Acad Sci U S A. 1989 Jun;86(11):3939-43. doi: 10.1073/pnas.86.11.3939.

DOI:10.1073/pnas.86.11.3939
PMID:2542957
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC287363/
Abstract

We have studied structural changes in the simian virus 40 (SV40) replication origin induced by SV40 large tumor antigen (T antigen). T-antigen-induced changes in origin DNA conformation can be visualized as specific and discrete topologic changes in origin DNA minicircles. We discovered three origin-T-antigen complexes defined by changes in DNA linking number. These complexes probably reflected essential early steps in the initiation of DNA replication since their formation required DNA sequences that are necessary for DNA replication but do not affect T-antigen binding. There are striking parallels between the T antigen-origin interactions uncovered by this assay and the interactions between the DnaA, -B, and -C proteins and the Escherichia coli replication origin, suggesting a significant evolutionary conservation in the mechanisms that initiate DNA replication.

摘要

我们研究了猿猴病毒40（SV40）大T抗原（T抗原）诱导的SV40复制起点的结构变化。T抗原诱导的起点DNA构象变化可表现为起点DNA小环中特定且离散的拓扑变化。我们发现了由DNA连环数变化定义的三种起点-T抗原复合物。这些复合物可能反映了DNA复制起始过程中必不可少的早期步骤，因为它们的形成需要DNA复制所必需但不影响T抗原结合的DNA序列。该实验揭示的T抗原与起点的相互作用，与DnaA、DnaB和DnaC蛋白与大肠杆菌复制起点之间的相互作用存在显著相似之处，这表明DNA复制起始机制具有重要的进化保守性。

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本文引用的文献

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Energetics of DNA twisting. II. Topoisomer analysis.DNA 扭曲的能量学。II. 拓扑异构体分析。
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