Pari G S, St Jeor S C
Department of Microbiology, University of Nevada, Reno 89557-0046.
Virology. 1990 Aug;177(2):824-8. doi: 10.1016/0042-6822(90)90558-9.
Human cytomegalovirus (HCMV) induces replication of the cloned SV40 origin of DNA replication in both productive and nonproductive infections. HCMV-induced replication of the SV40 DNA origin required the presence of T antigen. Human embryonic lung (HEL) cells were found to be fully permissive for SV40 origin replication only in the presence of HCMV gene expression. In addition, expression of plasmid encoded SV40 T antigen in HEL cells was only induced in the presence of HCMV. Cotransfection of the SV40-cloned origin of replication and seven overlapping cosmids containing the entire HCMV genome is capable of stimulating replication of the SV40 origin. We conclude that induction of the SV40 origin of replication by HCMV is (i) not due to any component of the HCMV virion, (ii) has a requirement for T antigen expression, (iii) occurs in an SV40 nonproductive cell, when T antigen expression is induced by HCMV gene expression.
人巨细胞病毒（HCMV）在增殖性感染和非增殖性感染中均可诱导克隆的SV40 DNA复制起点进行复制。HCMV诱导的SV40 DNA起点复制需要T抗原的存在。仅在HCMV基因表达存在时，人胚肺（HEL）细胞才被发现对SV40起点复制完全许可。此外，仅在HCMV存在时，HEL细胞中质粒编码的SV40 T抗原的表达才会被诱导。SV40克隆的复制起点与包含完整HCMV基因组的七个重叠黏粒共转染能够刺激SV40起点的复制。我们得出结论，HCMV对SV40复制起点的诱导（i）不是由于HCMV病毒体的任何成分，（ii）需要T抗原表达，（iii）当T抗原表达由HCMV基因表达诱导时，在SV40非增殖性细胞中发生。
