Lee Se Jin, Yu Jeong Seon, Parker Bruce L, Skinner Margaret, Je Yeon Ho, Kim Jae Su
Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea.
J Ind Microbiol Biotechnol. 2015 Jan;42(1):151-6. doi: 10.1007/s10295-014-1551-z. Epub 2014 Nov 28.
Efforts are underway to produce antimicrobial peptides in yellow mealworms (Tenebrio molitor), which can be developed as more effective and safer animal feed additives. In this work, we expressed Bombyx mori (Bm) cecropin-A in mealworms by the infection of transformed entomopathogenic Beauveria bassiana ERL1170. The active domain of Bm cecropin A gene was tagged with a signal sequence of B. bassiana for extracellular secretion, and the fragment was inserted into ERL1170 by the restriction enzyme-mediated integration method. Transformant D-6 showed antibacterial activity against Bacillus subtilis and Listeria monocytogenes. Against T. molitor larvae, D-6 had similar mortality to wild-type, and D6-infected mealworm suspension showed strong antibacterial activity against the two bacteria, but not in the wild-type-infected mealworms, thereby increasing the value of mealworms as animal feed additives.
人们正在努力利用黄粉虫（黄粉虫）生产抗菌肽，其可被开发为更有效、更安全的动物饲料添加剂。在这项工作中，我们通过感染转化的昆虫病原球孢白僵菌ERL1170，在黄粉虫中表达家蚕（Bm）天蚕素-A。Bm天蚕素A基因的活性结构域用球孢白僵菌的信号序列进行标记以进行细胞外分泌，该片段通过限制酶介导的整合方法插入ERL1170中。转化体D-6对枯草芽孢杆菌和单核细胞增生李斯特菌具有抗菌活性。对于黄粉虫幼虫，D-6与野生型具有相似的死亡率，并且D6感染的黄粉虫悬浮液对这两种细菌显示出很强的抗菌活性，但在野生型感染的黄粉虫中则没有，从而提高了黄粉虫作为动物饲料添加剂的价值。
