Schuschke D A, Saari J T
Department of Physiology, School of Medicine, University of North Dakota, Grand Forks.
Pharmacology. 1989;38(1):23-33. doi: 10.1159/000138514.
The role of adenosine 3',5'-monophosphate (cAMP) in histamine-mediated vasomotion of coronary vascular smooth muscle was studied in the isolated perfused rabbit heart. In paired physiological studies, histamine-mediated vasoconstriction, as indicated by change in perfusion pressure, was inhibited by the presence of either theophylline, a phosphodiesterase inhibitor, or forskolin, an adenylate cyclase activator. The inhibitory effect of theophylline, but not of forskolin, was removed with cimetidine (H2-receptor antagonist). In biochemical studies, coronary vessel cAMP was measured immediately after, and compared to, the vasomotor response to histamine alone and to histamine in the presence of forskolin, theophylline, diphenhydramine (H1-receptor antagonist) or cimetidine. These studies showed that cAMP levels correlate inversely with the vasoconstrictor response to histamine, indicating that stimulation of H1-receptors is associated with a reduction of cAMP and that H2-receptors modify this reduction.
在离体灌注兔心脏中研究了3',5'-环磷酸腺苷（cAMP）在组胺介导的冠状血管平滑肌血管运动中的作用。在配对的生理学研究中，磷酸二酯酶抑制剂茶碱或腺苷酸环化酶激活剂福斯高林的存在可抑制组胺介导的血管收缩（以灌注压力变化表示）。西咪替丁（H2受体拮抗剂）可消除茶碱而非福斯高林的抑制作用。在生化研究中，在对单独组胺以及组胺与福斯高林、茶碱、苯海拉明（H1受体拮抗剂）或西咪替丁共同作用后的血管运动反应后，立即测量冠状血管中的cAMP，并进行比较。这些研究表明，cAMP水平与对组胺的血管收缩反应呈负相关，表明H1受体的刺激与cAMP的降低有关，且H2受体可改变这种降低。
