Wu Wanshui, Jia Yongrui, Du Shuxu, Tang Hong, Sun Yangling, Sun Liming
Department of Pediatrics, Shijitan Hospital, Capital Medical University, Beijing 100038, China.
Health Science Center, Peking University, Beijing 100191, China.
Chin Med J (Engl). 2014;127(23):4110-3.
Bacterial inflammation is a common complication in patients with leukemia, and sulfur dioxide (SO2) is a bioactive molecule in modulating Gram-negative bacilli infection. This study aimed to examine the changes in SO2, nuclear factor-κB (NF-κB), and interleukin-8 (IL-8) levels in pediatric acute lymphoblastic leukemia (ALL) with Gram-negative bacterial inflammation.
Fifty-five ALL children were enrolled in this study, including 30 males and 25 females, aged 3-13 years, and the median age was 7.8 years. All these children who accepted chemotherapy for ALL were divided into the control group (before chemotherapy), the infection group (after chemotherapy with infection), and the recovery group (the infection was controlled after 1 week). The serum level of SO2 was detected using high performance liquid chromatography with fluorescence assay, and NF-κB and IL-8 levels were measured by enzyme-linked immunosorbent assay (ELISA). Human THP-1 cells were cultured, induced, and differentiated into macrophages, which were divided into five groups and each group was cultured with different stimulators: lipopolysaccharide (LPS) group, LPS+L-aspartate-β-hydroxamate (HDX) group, LPS+SO2 group, SO2, and control groups. NF-κB level and IL-8 protein contents by ELISA were examined in each group.
In comparison with those of the control group, levels of serum SO2, NF-κB, and IL-8 of the infection group were significantly increased (P < 0.05), while those of the recovery group were significantly decreased (P < 0.05). A positive correlation was found between levels of serum SO2 and intracellular NF-κB/IL-8, and the correlation coefficients were 0.671 and 0.798 (P < 0.05), respectively. According to the results found in human THP-1 cells, levels of NF-κB and IL-8 in LPS group were significantly increased compared with those of the control group (P < 0.05); when compared with those in LPS group, levels of NF-κB in LPS+HDX group further increased significantly (P < 0.05); however, the NF-κB levels of LPS+SO2 group decreased significantly (P < 0.05).
SO2 may play an anti-inflammatory role during the process of inflammation by inhibiting the activation and transcription of NF-κB.
细菌感染引发的炎症是白血病患者常见的并发症，而二氧化硫（SO₂）是一种可调节革兰氏阴性杆菌感染的生物活性分子。本研究旨在检测小儿急性淋巴细胞白血病（ALL）合并革兰氏阴性菌感染时二氧化硫、核因子-κB（NF-κB）及白细胞介素-8（IL-8）水平的变化。
选取55例ALL患儿，其中男30例，女25例，年龄3 - 13岁，中位年龄7.8岁。所有接受ALL化疗的患儿分为对照组（化疗前）、感染组（化疗后合并感染）和恢复组（感染1周后得到控制）。采用高效液相色谱荧光分析法检测血清SO₂水平，采用酶联免疫吸附测定法（ELISA）检测NF-κB和IL-8水平。培养人THP-1细胞，诱导分化为巨噬细胞，分为五组，每组用不同刺激物培养：脂多糖（LPS）组、LPS + L - 天冬氨酸 - β - 异羟肟酸（HDX）组、LPS + SO₂组、SO₂组和对照组。用ELISA检测每组NF-κB水平和IL-8蛋白含量。
与对照组相比，感染组血清SO₂、NF-κB和IL-8水平显著升高（P < 0.05），而恢复组则显著降低（P < 0.05）。血清SO₂水平与细胞内NF-κB/IL-8水平呈正相关，相关系数分别为0.671和0.798（P < 0.05）。根据人THP-1细胞实验结果，LPS组NF-κB和IL-8水平较对照组显著升高（P < 0.05）；与LPS组相比，LPS + HDX组NF-κB水平进一步显著升高（P < 0.05）；然而，LPS + SO₂组NF-κB水平显著降低（P < 0.05）。
SO₂可能通过抑制NF-κB的激活和转录在炎症过程中发挥抗炎作用。
