在退化的小鼠视网膜中使用Affymetrix标准微阵列基因芯片（169格式）进行基因组分析。

Genomic analysis using Affymetrix standard microarray genechips (169 format) in degenerate murine retina.

作者信息

Chung Sook Hyun, Shen Weiyong, Gillies Mark

机构信息

Macular Research Group, Clinical Ophthalmology and Eye Health, Sydney Eye Hospital, The University of Sydney, Level 2 South Block, Hospital Road, Sydney, NSW, 2000, Australia,

出版信息

Methods Mol Biol. 2015;1254:129-40. doi: 10.1007/978-1-4939-2152-2_10.

DOI:10.1007/978-1-4939-2152-2_10

PMID:25431062

Abstract

Microarray is one of the most useful tools for gene expression profiling. The growing development of microarray genechip technology has enabled increasingly sophisticated studies on the differences in gene transcription between diseased and non-diseased tissues and provides clues to their contribution to the disease in a single experiment. Thus, microarray is used in not only in clinical diagnostics, but also to understand pathological processes and identify leads for new treatments. Here, we present a detailed protocol for performing genomic analysis of retinal tissue with Affymetrix genechip microarray together with additional guidelines from the authors.

摘要

微阵列是基因表达谱分析最有用的工具之一。微阵列基因芯片技术的不断发展，使得对患病组织和非患病组织之间基因转录差异进行越来越复杂的研究成为可能，并在一次实验中为它们对疾病的影响提供线索。因此，微阵列不仅用于临床诊断，还用于了解病理过程和确定新治疗方法的线索。在此，我们提供了一份使用Affymetrix基因芯片微阵列对视网膜组织进行基因组分析的详细方案以及作者的其他指导原则。