基于热敏共聚物水凝胶的干细胞移植介导的梗死心肌修复增强
Enhanced infarct myocardium repair mediated by thermosensitive copolymer hydrogel-based stem cell transplantation.
作者信息
Xia Yu, Zhu Kai, Lai Hao, Lang Meidong, Xiao Yan, Lian Sheng, Guo Changfa, Wang Chunsheng
机构信息
Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P. R. China Department of Cardio-Thoracic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, P. R. China.
Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China Shanghai Institute of Cardiovascular Disease, Shanghai 200032, P. R. China.
出版信息
Exp Biol Med (Maywood). 2015 May;240(5):593-600. doi: 10.1177/1535370214560957. Epub 2014 Nov 27.
Mesenchymal stem cell (MSC) transplantation by intramyocardial injection has been proposed as a promising therapy strategy for cardiac repair after myocardium infarction. However, low retention and survival of grafted MSCs hinder its further application. In this study, copolymer with N-isopropylacrylamide/acrylic acid/2-hydroxylethyl methacrylate-poly(ɛ-caprolactone) ratio of 88:9.6:2.4 was bioconjugated with type I collagen to construct a novel injectable thermosensitive hydrogel. The injectable and biocompatible hydrogel-mediated MSC transplantation could enhance the grafted cell survival in the myocardium, which contributed to the increased neovascularization, decreased interstitial fibrosis, and ultimately improved heart function to a significantly greater degree than regular MSC transplantation. We suggest that this novel hydrogel has the potential for future stem cell transplantation.
心肌内注射间充质干细胞（MSC）已被提议作为心肌梗死后心脏修复的一种有前景的治疗策略。然而，移植的MSC保留率低和存活率低阻碍了其进一步应用。在本研究中，将N - 异丙基丙烯酰胺/丙烯酸/甲基丙烯酸2 - 羟乙酯 - 聚（ε - 己内酯）比例为88:9.6:2.4的共聚物与I型胶原进行生物共轭，构建了一种新型的可注射热敏水凝胶。这种可注射且具有生物相容性的水凝胶介导的MSC移植可提高移植细胞在心肌中的存活率，这有助于增加新生血管形成、减少间质纤维化，并最终比常规MSC移植在更大程度上显著改善心脏功能。我们认为这种新型水凝胶在未来干细胞移植方面具有潜力。
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