Kim Chan Woo, Kim Chan Joon, Park Eun-Hye, Lee Eunmi, Seong Eunhwa, Chang Kiyuk
Cardiovascular Research Institute for Intractable Disease, College of Medicine, The Catholic University of Korea; Division of Cardiology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea.
Cardiovascular Research Institute for Intractable Disease, College of Medicine, The Catholic University of Korea; Division of Cardiology, Department of Internal Medicine, Uijeongbu St. Mary's Hospital, College of Medicine, The Catholic University of Korea;
J Vis Exp. 2020 Sep 20(163). doi: 10.3791/61752.
One of the major issues facing current cardiac stem cell therapies for preventing postinfarct heart failure is the low retention and survival rates of transplanted cells within the injured myocardium, limiting their therapeutic efficacy. Recently, the use of scaffolding biomaterials has gained attention for improving and maximizing stem cell therapy. The objective of this protocol is to introduce a simple and straightforward technique to transplant bone marrow-derived mesenchymal stem cells (MSCs) using injectable hydroxyphenyl propionic acid (GH) hydrogels; the hydrogels are favorable as a cell delivery platform for cardiac tissue engineering applications due to their ability to be cross-linked in situ and high biocompatibility. We present a simple method to fabricate MSC-loading GH hydrogels (MSC/hydrogels) and evaluate their survival and proliferation in three-dimensional (3D) in vitro culture. In addition, we demonstrate a technique for intramyocardial transplantation of MSC/hydrogels in mice, describing a surgical procedure to induce myocardial infarction (MI) via left anterior descending (LAD) coronary artery ligation and subsequent MSC/hydrogels transplantation.
当前用于预防心肌梗死后心力衰竭的心脏干细胞疗法面临的主要问题之一是移植细胞在受损心肌内的低保留率和存活率，这限制了它们的治疗效果。最近，支架生物材料的使用因改善和最大化干细胞疗法而受到关注。本方案的目的是介绍一种简单直接的技术，使用可注射的羟基苯丙酸（GH）水凝胶移植骨髓间充质干细胞（MSC）；由于其能够原位交联和具有高生物相容性，这些水凝胶作为心脏组织工程应用的细胞递送平台具有优势。我们提出了一种制备负载MSC的GH水凝胶（MSC/水凝胶）的简单方法，并评估它们在三维（3D）体外培养中的存活和增殖情况。此外，我们展示了一种在小鼠体内进行心肌内移植MSC/水凝胶的技术，描述了通过左前降支（LAD）冠状动脉结扎诱导心肌梗死（MI）并随后进行MSC/水凝胶移植的手术过程。
