两种不同葡萄糖浓度对人肝癌HepG2细胞中胰岛素受体mRNA水平的影响。
Effect of two different glucose concentrations on insulin receptor mRNA levels in human hepatoma HepG2 cells.
作者信息
Briata P, Gherzi R, Adezati L, Cordera R
机构信息
Department of Internal Medicine, University of Genova, Italy.
出版信息
Biochem Biophys Res Commun. 1989 May 15;160(3):1415-20. doi: 10.1016/s0006-291x(89)80162-7.
Glucose is known to affect mRNA levels of several genes. In order to investigate possible effects of glucose on insulin receptor mRNA levels, we cultured human hepatoma cells (HepG2) in two different culture media: DMEM containing 100 mg/dl glucose and DMEM containing 450 mg/dl glucose. Insulin receptor mRNA levels and insulin binding activity were reduced in HepG2 cultured at lower glucose concentrations. These data suggest that glucose affects insulin receptor gene expression.
已知葡萄糖会影响多个基因的mRNA水平。为了研究葡萄糖对胰岛素受体mRNA水平可能产生的影响，我们在两种不同的培养基中培养人肝癌细胞（HepG2）：含有100mg/dl葡萄糖的DMEM和含有450mg/dl葡萄糖的DMEM。在较低葡萄糖浓度下培养的HepG2细胞中，胰岛素受体mRNA水平和胰岛素结合活性降低。这些数据表明葡萄糖会影响胰岛素受体基因的表达。
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