Xie Hui-Chun, Shang Jun
The key laboratory of education ministry on environments and resources in Tibetan Plateau, Qinghai-Normal University, Xining 810008, China.
Afr J Tradit Complement Altern Med. 2014 Jan 28;11(2):358-62. doi: 10.4314/ajtcam.v11i2.22. eCollection 2014.
Radix et Rhizoma Rhei has a gastric mucosal protective effect, major anti-gastritis and anti-peptic ulcer active constituents were emodin, aloe-emodin, chrysophanol, rhein, etc. The objective of the study was the extraction process of total anthraquinones in Radix et Rhizoma rhei and their antilipemic effects.
Orthogonal experiment, UV spectrophotometry and conventional antilipemic effect determination method were used to optimize the extraction process, and to determine the total anthraquinone content, as well as blood levels of total cholesterol, triglycerides, HDL and LDL.
Ethanol concentration, extraction time and ethanol amount had significant influences on the extraction of total Radix et Rhizoma rhei anthraquinones, total Radix et Rhizoma rhei anthraquinones could significantly reduce blood levels of total cholesterol, triglycerides, HDL and LDL.
The optimum extraction process was two times extraction of Radix et Rhizoma rhei with 70% ethanol, the amounts of solvent of 8 folds and 5 folds, successively, and the extraction time of 60 min each. In addition, this extract has an antilipemic effect in mice.
大黄具有胃黏膜保护作用，主要的抗胃炎和抗消化性溃疡活性成分有大黄素、芦荟大黄素、 Chrysophanol、大黄酸等。本研究的目的是大黄总蒽醌的提取工艺及其降血脂作用。
采用正交试验、紫外分光光度法和传统的降血脂作用测定方法优化提取工艺，测定总蒽醌含量以及总胆固醇、甘油三酯、高密度脂蛋白和低密度脂蛋白的血药浓度。
乙醇浓度、提取时间和乙醇用量对大黄总蒽醌的提取有显著影响，大黄总蒽醌能显著降低总胆固醇、甘油三酯、高密度脂蛋白和低密度脂蛋白的血药浓度。
最佳提取工艺为用70%乙醇对大黄进行两次提取，溶剂用量依次为8倍量和5倍量，每次提取时间为60分钟。此外，该提取物对小鼠有降血脂作用。
