氨基酸谱作为测定果汁真伪的可行工具。
Amino Acid profile as a feasible tool for determination of the authenticity of fruit juices.
作者信息
Asadpoor Mostafa, Ansarin Masoud, Nemati Mahboob
机构信息
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Students' Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
Adv Pharm Bull. 2014 Dec;4(4):359-62. doi: 10.5681/apb.2014.052. Epub 2014 Aug 10.
PURPOSE
Fruit juice is a nutrient rich food product with a direct connection to public health. The purpose of this research was to determine the amino acid profile of juices and provide a quick and accurate indicator for determining their authenticity.
METHODS
The method of analysis was HPLC with fluorescence detector and pre-column derivatization by orthophtaldialdehyde (OPA). Sixty-six samples of fruit juices were analyzed, and fourteen amino acids were identified and determined in the sampled fruit juices. The fruit samples used for this analysis were apples, oranges, cherry, pineapple, mango, apricot, pomegranate, peach and grapes.
RESULTS
The results showed that 32% of samples tested in this study had a lower concentrate percentage as compared to that of their labels and/or other possible authenticity problems in the manufacturing process. The following samples showed probable adulteration: four cherry juice samples, two pomegranate juice samples, one mango, three grape, four peach, seven orange, two apple and one apricot juice samples.
CONCLUSION
In general, determining the amount of amino acids and comparing sample amino acids profiles with the standard values seems to be an indicator for quality control. This method can provide the regulatory agencies with a tool, to help produce a healthier juice. The aim of this study is the analytical control of the fruit juice composition is becoming an important issue, and HPLC can provide an important and essential tool for more accurate research as well as for routine analysis.
目的
果汁是一种营养丰富的食品，与公众健康直接相关。本研究的目的是确定果汁的氨基酸谱，并提供一种快速准确的指标来确定其真伪。
方法
分析方法为采用荧光检测器的高效液相色谱法（HPLC），并通过邻苯二甲醛（OPA）进行柱前衍生化。分析了66个果汁样品，在所采集的果汁中鉴定并测定了14种氨基酸。用于该分析的水果样品有苹果、橙子、樱桃、菠萝、芒果、杏、石榴、桃子和葡萄。
结果
结果表明，本研究中测试的32%的样品的浓缩汁百分比低于其标签标注的百分比，和/或在生产过程中存在其他可能的真伪问题。以下样品显示可能存在掺假情况：4个樱桃汁样品、2个石榴汁样品、1个芒果汁样品、3个葡萄汁样品、4个桃汁样品、7个橙汁样品、2个苹果汁样品和1个杏汁样品。
结论
总体而言，测定氨基酸含量并将样品氨基酸谱与标准值进行比较似乎是质量控制的一个指标。该方法可为监管机构提供一种工具，以帮助生产更健康的果汁。本研究的目的是对果汁成分进行分析控制正成为一个重要问题，而高效液相色谱法可为更准确的研究以及常规分析提供重要且必要的工具。
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