Rodrigues de Santana Fabiana, de Paula Coelho Cidéli, Cardoso Thayná Neves, Perez Hurtado Elizabeth Cristina, Roberti Benites Nilson, Dalastra Laurenti Marcia, Villano Bonamin Leoni
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil.
Graduate Program in Environmental and Experimental Pathology, Research Center of University Paulista, Rua Dr Bacelar, 1212 - 4th Floor, 04026-002 São Paulo, Brazil; Laboratory of Veterinary Pathology, University of Santo Amaro, São Paulo, Brazil.
Homeopathy. 2014 Oct;103(4):264-74. doi: 10.1016/j.homp.2014.08.006. Epub 2014 Oct 3.
Leishmaniasis is a zoonotic disease caused by protozoan parasites of the mononuclear phagocytic system. The modulation activity of these cells can interfere in the host/parasite relationship and influences the prognosis.
We evaluated the effects of the homeopathic preparation Antimonium crudum 30cH on experimental infection induced by Leishmania (L.) amazonensis. Male Balb/c mice were inoculated with 2 × 10(6)Leishmania (L.) amazonensis promastigotes into the footpad and, after 48 h (acute phase) or 60 days (chronic phase), cell population of lymphocytes and phagocytes present in the peritoneal washing fluid and spleen were analyzed by flow cytometry and histopathology, with histometry of the subcutaneous primary lesion, local lymph node and spleen. Immunohistochemistry was performed to quantify CD3 (T lymphocyte), CD45RA (B lymphocyte) and CD11b (phagocytes) positive cells.
In treated mice, during the acute phase, there was significant increase of the macroscopic lesion, associated to inflammatory edema, as well increase in the number of free amastigotes and B lymphocytes inside the lesion. Increase of B lymphocytes (predominantly B-2 cells) was also seen in the local lymph node, spleen and peritoneum. In the chronic phase, the inflammatory process in the infection focus was reduced, with reduced phagocyte migration and peritoneal increase of B-1a cells (precursors of B-2 immunoglobulin producers cells) and T CD8+ cells.
The treatment of mice with Antimonium crudum 30cH induced a predominantly B cell pattern of immune response in Leishmania (L.) amazonensis experimental infection, alongside the increase of free amastigote forms number in the infection site. The clinical significance of this study is discussed, further studies are suggested.
利什曼病是一种由单核吞噬系统的原生动物寄生虫引起的人畜共患病。这些细胞的调节活性可干扰宿主/寄生虫关系并影响预后。
我们评估了顺势疗法制剂30cH粗制锑对亚马逊利什曼原虫诱导的实验性感染的影响。将雄性Balb/c小鼠的足垫接种2×10⁶亚马逊利什曼原虫前鞭毛体，在48小时（急性期）或60天（慢性期）后，通过流式细胞术和组织病理学分析腹膜冲洗液和脾脏中淋巴细胞和吞噬细胞的细胞群体，并对皮下原发性病变、局部淋巴结和脾脏进行组织计量分析。进行免疫组织化学以量化CD3（T淋巴细胞）、CD45RA（B淋巴细胞）和CD11b（吞噬细胞）阳性细胞。
在治疗的小鼠中，急性期时，宏观病变显著增加，伴有炎性水肿，病变内游离无鞭毛体和B淋巴细胞数量也增加。在局部淋巴结、脾脏和腹膜中也观察到B淋巴细胞（主要是B-2细胞）增加。在慢性期，感染灶的炎症过程减轻，吞噬细胞迁移减少，B-1a细胞（B-2免疫球蛋白产生细胞的前体）和T CD8⁺细胞在腹膜中增加。
用30cH粗制锑治疗小鼠在亚马逊利什曼原虫实验性感染中诱导了以B细胞为主的免疫反应模式，同时感染部位游离无鞭毛体形式的数量增加。讨论了本研究的临床意义，并提出了进一步的研究建议。
