细胞色素c导入线粒体：由NADH和黄素核苷酸介导的血红素还原，是其与脱辅基细胞色素c共价连接所必需的。
Import of cytochrome c into mitochondria: reduction of heme, mediated by NADH and flavin nucleotides, is obligatory for its covalent linkage to apocytochrome c.
作者信息
Nicholson D W, Neupert W
机构信息
Institut für Physiologische Chemie, Universität München, Federal Republic of Germany.
出版信息
Proc Natl Acad Sci U S A. 1989 Jun;86(12):4340-4. doi: 10.1073/pnas.86.12.4340.
Abstract
The covalent attachment of heme to apocytochrome c, and therefore the import of cytochrome c into mitochondria, is dependent on both NADH plus a cytosolic cofactor that has been identified to be FMN or FAD. NADH in concert with flavin nucleotides mediates the reduction of heme. Heme in the reduced state is a prerequisite for its covalent attachment to apocytochrome c by the enzyme cytochrome c heme lyase and thus for subsequent translocation of cytochrome c across the outer mitochondrial membrane during import.
摘要
血红素与脱辅基细胞色素c的共价连接，进而细胞色素c导入线粒体，依赖于NADH以及一种已被鉴定为FMN或FAD的胞质辅因子。NADH与黄素核苷酸协同介导血红素的还原。还原态的血红素是其通过细胞色素c血红素裂解酶与脱辅基细胞色素c共价连接的前提条件，因此也是细胞色素c在导入过程中跨线粒体外膜转运的前提条件。
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本文引用的文献
1
Genetics and biosynthesis of cytochrome c.细胞色素c的遗传学与生物合成
Annu Rev Genet. 1971;5:257-96. doi: 10.1146/annurev.ge.05.120171.001353.
2
Biogenesis of cytochrome c in Neurospora crassa.粗糙脉孢菌中细胞色素c的生物合成
Methods Enzymol. 1983;97:261-74. doi: 10.1016/0076-6879(83)97138-0.
3
Receptor sites involved in posttranslational transport of apocytochrome c into mitochondria: specificity, affinity, and number of sites.参与脱辅基细胞色素c向线粒体进行翻译后转运的受体位点：特异性、亲和力及位点数量
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4963-7. doi: 10.1073/pnas.80.16.4963.
4
5
6
In vitro synthesis and posttranslational uptake of cytochrome c into isolated mitochondria: role of a specific addressing signal in the apocytochrome.细胞色素c在体外合成及翻译后进入分离线粒体的过程：脱辅基细胞色素中特定寻址信号的作用
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4368-72. doi: 10.1073/pnas.78.7.4368.
7
Formation of a cytochrome c-like species from horse apoprotein and hemin catalyzed by yeast mitochondrial cytochrome c synthetase.酵母线粒体细胞色素c合成酶催化马脱辅基蛋白和血红素形成细胞色素c样物质。
J Biol Chem. 1981 Aug 25;256(16):8646-51.
8
Different transport pathways of individual precursor proteins in mitochondria.线粒体中单个前体蛋白的不同转运途径。
Eur J Biochem. 1981 Jun 1;116(3):455-60. doi: 10.1111/j.1432-1033.1981.tb05357.x.
9
Identification and mutational relocation of the AUG codon initiating translation of iso-1-cytochrome c in yeast.酵母中起始异-1-细胞色素c翻译的AUG密码子的鉴定及突变重定位
J Biol Chem. 1971 Dec 25;246(24):7429-45.
10
Association of ferrochelatase with Complex I in bovine heart mitochondria.牛心线粒体中铁螯合酶与复合体I的关联。
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