Maruwaka Mikio, Yoshikawa Kazuhiro, Okamoto Sho, Araki Yoshio, Sumitomo Masaki, Kawamura Akino, Yokoyama Kinya, Wakabayashi Toshihiko
Department of Neurosurgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Division of Advanced Research Promotion Institute of Comprehensive Medical Research, Nagakute-City, Japan; Division of Advanced Medicine Advanced Medical Research Center; Laboratory of Cell Therapy Division of Advanced Medical Research Center.
J Stroke Cerebrovasc Dis. 2015 Jan;24(1):104-11. doi: 10.1016/j.jstrokecerebrovasdis.2014.07.028. Epub 2014 Oct 28.
Moyamoya disease (MMD) is a rare cerebrovascular disease characterized by steno-occlusive change in bilateral internal carotid arteries with unknown etiology. To discover biomarker candidates in cerebrospinal fluid from MMD patients, proteome analysis was performed by the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Three peptides, 4473Da, 4475Da, and 6253Da, were significantly elevated in MMD group. A positive correlation between 4473Da peptide and postoperative angiogenesis was determined. Twenty MMD patients were enrolled in this pilot study, including 11 pediatric cases less than 18 years of age (mean age, 8.67 years) and 9 adult MMD patients (mean age, 38.1 years). This study also includes 17 control cases with the mean age of 27.9 years old. In conclusion, 4473Da peptide is supposed to be a reliable biomarker of MMD. 4473Da peptide showed higher intensity peaks especially in younger MMD patients, and it was proved to be highly related to postoperative angiogenesis. Further study is needed to show how 4473Da peptide is involved with the etiology and the onset of MMD.
烟雾病（MMD）是一种罕见的脑血管疾病，其特征为双侧颈内动脉出现狭窄闭塞性改变，病因不明。为了在烟雾病患者的脑脊液中发现潜在的生物标志物，采用表面增强激光解吸/电离飞行时间质谱法进行了蛋白质组分析。在烟雾病组中，三种肽段（4473Da、4475Da和6253Da）显著升高。确定了4473Da肽段与术后血管生成之间存在正相关。本前瞻性研究纳入了20例烟雾病患者，其中包括11例18岁以下的儿童病例（平均年龄8.67岁）和9例成年烟雾病患者（平均年龄38.1岁）。本研究还包括17例平均年龄为27.9岁的对照病例。总之，4473Da肽段被认为是烟雾病的一种可靠生物标志物。4473Da肽段尤其在年轻烟雾病患者中显示出更高强度的峰，并且已证明其与术后血管生成高度相关。需要进一步研究以揭示4473Da肽段如何参与烟雾病的病因和发病过程。
