肩突硬蜱线粒体16S核糖体RNA基因的序列及二级结构

Sequence and secondary structure of the mitochondrial 16S ribosomal RNA gene of Ixodes scapularis.

作者信息

Krakowetz Chantel N, Chilton Neil B

机构信息

Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5EZ, Canada.

出版信息

Mol Cell Probes. 2015 Feb;29(1):35-8. doi: 10.1016/j.mcp.2014.11.001. Epub 2014 Nov 15.

DOI:10.1016/j.mcp.2014.11.001

PMID:25444935

Abstract

The complete DNA sequences and secondary structure of the mitochondrial (mt) 16S ribosomal (r) RNA gene were determined for six Ixodes scapularis adults. There were 44 variable nucleotide positions in the 1252 bp sequence alignment. Most (95%) nucleotide alterations did not affect the integrity of the secondary structure of the gene because they either occurred at unpaired positions or represented compensatory changes that maintained the base pairing in helices. A large proportion (75%) of the intraspecific variation in DNA sequence occurred within Domains I, II and VI of the 16S gene. Therefore, several regions within this gene may be highly informative for studies of the population genetics and phylogeography of I. scapularis, a major vector of pathogens of humans and domestic animals in North America.

摘要

测定了6只肩突硬蜱成虫线粒体（mt）16S核糖体（r）RNA基因的完整DNA序列和二级结构。在1252bp的序列比对中有44个可变核苷酸位点。大多数（95%）核苷酸改变不影响基因二级结构的完整性，因为它们要么发生在未配对位置，要么代表维持螺旋中碱基配对的补偿性变化。16S基因DNA序列种内变异的很大一部分（75%）发生在该基因的结构域I、II和VI内。因此，该基因内的几个区域可能对北美人类和家畜主要病原体传播媒介肩突硬蜱的群体遗传学和系统地理学研究具有高度信息价值。