骨形态发生蛋白（BMP）和视黄酸（RA）信号通路在胚胎发育过程中协同调节载脂蛋白C1的表达。

BMP and RA signaling cooperate to regulate Apolipoprotein C1 expression during embryonic development.

作者信息

Wang Yang, Li Wen-Hua, Li Zhi, Liu Wei, Zhou Li, Gui Jian-Fang

机构信息

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China; University of the Chinese Academy of Sciences, Wuhan, China.

出版信息

Gene. 2015 Jan 10;554(2):196-204. doi: 10.1016/j.gene.2014.10.047. Epub 2014 Oct 28.

DOI:10.1016/j.gene.2014.10.047

PMID:25445289

Abstract

Apolipoproteins, the major components of lipoproteins, play physiological roles in lipoprotein metabolism. Contrary to the well-documented effects on plasma lipid, little is known about the function and regulation of Apolipoproteins during embryonic development. Here we have shown that apolipoprotein C1 (apoc1) gene is highly expressed in the yolk syncytial layer, a structure implicated in embryonic and larval nutrition. The apoc1 transcripts are also observed in the deep cell layer at the ventral and lateral region during gastrulation, and in the tail paraxial mesoderm during somitogenesis. By whole-mount in situ hybridization and quantified RT-RCR, we further demonstrate that apoc1 expression is induced by bone morphogenetic proteins (BMPs) signaling, while retinoic acid (RA) signaling suppresses the expression of BMP ligands and inhibits the BMP effect in this process.

摘要

载脂蛋白是脂蛋白的主要成分，在脂蛋白代谢中发挥生理作用。与对血浆脂质的充分记录的影响相反，关于载脂蛋白在胚胎发育过程中的功能和调节知之甚少。在这里，我们表明载脂蛋白C1（apoc1）基因在卵黄合胞体层中高度表达，卵黄合胞体层是一种与胚胎和幼体营养有关的结构。在原肠胚形成期间，在腹侧和外侧区域的深层细胞层中也观察到apoc1转录本，在体节形成期间在尾旁中胚层中也观察到apoc1转录本。通过全胚胎原位杂交和定量RT-RCR，我们进一步证明apoc1表达是由骨形态发生蛋白（BMP）信号诱导的，而视黄酸（RA）信号抑制BMP配体的表达并在此过程中抑制BMP的作用。