State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Sci China Life Sci. 2013 Nov;56(11):975-84. doi: 10.1007/s11427-013-4563-4. Epub 2013 Nov 8.
Apolipoprotein C1 (Apoc1) is associated with lipoprotein metabolism, but its physiological role during embryogenesis is largely unknown. We reveal a new function of Apoc1b, a transcript isoform of Apoc1, in epiboly during zebrafish gastrulation. Apoc1b is expressed in yolk syncytial layers and in deep cells of the ventral and lateral region of the embryos. It displays a radial gradient with high levels in the interior layer and low levels in the superficial layer. Knockdown of Apoc1b by injecting antisense morpholino (MO) caused the epiboly arrest in deep cells. Moreover, we show that the radial intercalation and the radial gradient distribution of E-cadherin are disrupted both in Apoc1b knockdown and overexpressed embryos. Therefore, Apoc1b controls epiboly via E-cadherin-mediated radial intercalation in a gradient-dependent manner.
载脂蛋白 C1（Apoc1）与脂蛋白代谢有关，但在胚胎发生过程中其生理作用在很大程度上尚不清楚。我们揭示了 Apoc1 的一种新的转录本异构体 Apoc1b 在斑马鱼原肠胚形成中的胚环运动中的功能。Apoc1b 在卵黄合胞层和胚胎腹侧和外侧的深层细胞中表达。它呈现出一种径向梯度，内部层的水平高，而表面层的水平低。通过注射反义 morpholino（MO）敲低 Apoc1b 会导致深层细胞的胚环运动停止。此外，我们还表明，Apoc1b 敲低和过表达的胚胎中 E-cadherin 的放射状内插和放射状梯度分布均受到破坏。因此，Apoc1b 通过 E-cadherin 介导的梯度依赖性放射状内插来控制胚环运动。
