延髓背角中V谷氨酸转运体2与神经降压素或亮脑啡肽的共存：大鼠的共聚焦和电子显微镜免疫组织化学研究

The coexistence of VGluT2 and neurotensin or leu-enkephalin in the medullary dorsal horn: a confocal and electron microscopic immunohistochemical study in the rat.

作者信息

Liu Tao, Li Jinlian, Liu Hui, Wang Xin, Fan Feiyan, Zhang Pengxing, Tu Yanyang, Zhang Yongsheng

机构信息

Department of Anatomy, Histology and Embryology and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China; Department of Dermatology, Tangdu Hospital, The Fourth Military Medical University, Xi'an 710038, PR China.

Department of Anatomy, Histology and Embryology and K. K. Leung Brain Research Centre, The Fourth Military Medical University, Xi'an 710032, PR China.

出版信息

Neurosci Lett. 2015 Jan 1;584:390-4. doi: 10.1016/j.neulet.2014.11.008. Epub 2014 Nov 11.

DOI:10.1016/j.neulet.2014.11.008

PMID:25445367

Abstract

Neuropeptides such as neurotensin (NT), and enkephalin (ENK) in the medullary dorsal horn (MDH) are involved in excitatory synaptic transmission to modulate nociceptive information. However, morphological evidence indicating that NT or ENK coexists with glutamate in the MDH is still meager. Using fluorescent immunohistochemistry, the results showed that double labeling of NT or ENK terminals with VGluT2 is mainly concentrated in the lamina II of the MDH, and many axon terminals exhibiting NT or ENK immunoreactivity in the superficial layers of the MDH showed VGluT2 immunoreactivity. Electron microscopy confirmed the coexpression of NT or ENK and VGluT2 in axon terminals within the laminae I and II of the MDH. These axon terminals make asymmetrical synapses with immunonegative neuronal cell bodies and dendrites. The findings suggest that glutamate is coreleased with NT or ENK from axon terminals of interneurons in the superficial layers of the MDH.

摘要

延髓背角（MDH）中的神经肽，如神经降压素（NT）和脑啡肽（ENK），参与兴奋性突触传递以调节伤害性信息。然而，表明NT或ENK与谷氨酸在MDH中共存的形态学证据仍然不足。利用荧光免疫组织化学，结果显示NT或ENK终末与囊泡谷氨酸转运体2（VGluT2）的双重标记主要集中在MDH的Ⅱ层，并且在MDH浅层中许多呈现NT或ENK免疫反应性的轴突终末显示出VGluT2免疫反应性。电子显微镜证实了NT或ENK与VGluT2在MDH的Ⅰ层和Ⅱ层内的轴突终末中共表达。这些轴突终末与免疫阴性的神经元胞体和树突形成不对称突触。这些发现表明，谷氨酸在MDH浅层中间神经元的轴突终末与NT或ENK共同释放。