Kiyozumi Daiji, Sato-Nishiuchi Ryoko, Sekiguchi Kiyotoshi
Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka, Japan.
Curr Protoc Cell Biol. 2014 Dec 1;65:10.19.1-17. doi: 10.1002/0471143030.cb1019s65.
Integrins are cell surface receptors for cell adhesion. Integrin-mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobilized within extracellular matrices, insoluble sheet-like or fibrous supramolecular complexes that associate with or surround cells. To better understand the molecular basis of integrin-mediated regulation of cellular behavior in vivo, it is of critical importance to collect information regarding the activities as well as spatial distributions of integrin ligands in situ. This unit describes a protocol for detecting the spatial distribution of the complement of integrin ligands in situ by overlaying soluble recombinant integrins.
整合素是细胞表面的细胞黏附受体。整合素介导的细胞黏附调节多种细胞过程，包括细胞存活、迁移、增殖和分化。在体内，整合素的配体固定在细胞外基质中，细胞外基质是与细胞相关联或围绕细胞的不溶性片状或纤维状超分子复合物。为了更好地理解体内整合素介导的细胞行为调节的分子基础，收集有关整合素配体原位活性以及空间分布的信息至关重要。本单元描述了一种通过覆盖可溶性重组整合素来检测整合素配体原位互补物空间分布的方法。
