Polishchuk A G, Nevzgliadova O V, Tereshin I M, Soĭdla T R
Mol Biol (Mosk). 1989 Jan-Feb;23(1):279-85.
We propose a method for isolation of enhancer-like sequences from a yeast genomic library. The method was used to identify DNA inserts capable of increasing bacterial bla gene expression when located downstream from the transcription initiation site. Plasmids carrying different fragments of one such insert were used to localize the enhancer-like function on a 1.2 kb fragment.