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[Isolation of nucleotide sequences, possessing enhancer functions from the Saccharomyces cerevisiae genetic library].

作者信息

Polishchuk A G, Nevzgliadova O V, Tereshin I M, Soĭdla T R

出版信息

Mol Biol (Mosk). 1989 Jan-Feb;23(1):279-85.

PMID:2544796
Abstract

We propose a method for isolation of enhancer-like sequences from a yeast genomic library. The method was used to identify DNA inserts capable of increasing bacterial bla gene expression when located downstream from the transcription initiation site. Plasmids carrying different fragments of one such insert were used to localize the enhancer-like function on a 1.2 kb fragment.

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