Verstappen Koen M, Duim Birgitta, van Nes Arie, Snijders Susan, van Wamel Willem J B, Wagenaar Jaap A
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands.
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL Utrecht, The Netherlands.
Vet Microbiol. 2014 Dec 5;174(3-4):483-488. doi: 10.1016/j.vetmic.2014.09.019. Epub 2014 Oct 5.
Methicillin-resistant Staphylococcus aureus sequence type (ST)398 is widely spread among livestock. People in contact with livestock have a higher risk of testing positive for MRSA. Several experimental settings have been described to study in vivo colonization of MRSA in pigs, each having its own limitations. The aim of this study was to develop a nose-colonization model in pigs to quantitatively study the colonization of MRSA and the co-colonization of MSSA and MRSA. Two experiments were performed: in the first experiment piglets received an intranasal inoculation with MRSA ST398, spa-type t011, and in the second experiment piglets received an intranasal inoculation with two MSSA strains (ST398, spa-type t011 and t034) and two MRSA strains (also ST398, spa-type t011 and t034) to investigate co-colonization. Colonization was quantitatively monitored for 2 weeks in both experiments. Nasal colonization was successfully established in all piglets with stable numbers of S. aureus between 10(4) and 10(6) CFU. MSSA and MRSA were able to co-colonize.
耐甲氧西林金黄色葡萄球菌序列类型（ST）398在牲畜中广泛传播。与牲畜接触的人检测出耐甲氧西林金黄色葡萄球菌呈阳性的风险更高。已经描述了几种实验设置来研究耐甲氧西林金黄色葡萄球菌在猪体内的定植情况，每种设置都有其自身的局限性。本研究的目的是建立一种猪鼻定植模型，以定量研究耐甲氧西林金黄色葡萄球菌的定植以及甲氧西林敏感金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌的共定植情况。进行了两项实验：在第一项实验中，仔猪经鼻接种耐甲氧西林金黄色葡萄球菌ST398，spa型t011；在第二项实验中，仔猪经鼻接种两种甲氧西林敏感金黄色葡萄球菌菌株（ST398，spa型t011和t034）和两种耐甲氧西林金黄色葡萄球菌菌株（同样是ST398，spa型t011和t034）以研究共定植情况。在两项实验中，对定植情况进行了2周的定量监测。所有仔猪均成功建立鼻定植，金黄色葡萄球菌数量稳定在10⁴至10⁶CFU之间。甲氧西林敏感金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌能够共定植。
