İnci Duygu, Aydın Rahmiye, Yılmaz Dilek, Gençkal Hasene Mutlu, Vatan Özgür, Çinkılıç Nilüfer, Zorlu Yunus
Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Uludag University, 16059 Bursa, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Feb 5;136 Pt B:761-70. doi: 10.1016/j.saa.2014.09.093. Epub 2014 Sep 30.
Two new water-soluble copper(II) complexes, [Cu(dmphen)2(NO3)]NO3 (1), [Cu(dmphen)(tyr)(H2O)]NO3·H2O (2) and the diquarternary salt of dmphen (dmphen = 4,7-dimethyl-1,10-phenanthroline and tyr = L-tyrosine), have been synthesized and characterized by elemental analysis, (1)H NMR, (13)C NMR and IR spectroscopy, thermal analysis and single crystal X-ray diffraction techniques. The CT-DNA binding properties of these compounds have been investigated by absorption, emission spectroscopy and thermal denaturation measurements. The supercoiled pBR322 plasmid DNA cleavage activity of these compounds has been explored by agarose gel electrophoresis. The cytotoxicity of these compounds against MCF-7, Caco-2, A549 cancer cells and BEAS-2B healthy cells was also studied by the XTT method. Complexes 1 and 2 exhibit significant cytotoxicity, with lower IC50 values than those of cisplatin.
合成了两种新型水溶性铜（II）配合物[Cu(dmphen)2(NO3)]NO3 (1)、[Cu(dmphen)(tyr)(H2O)]NO3·H2O (2) 以及dmphen的二季铵盐（dmphen = 4,7-二甲基-1,10-菲咯啉，tyr = L-酪氨酸），并通过元素分析、1H NMR、13C NMR、红外光谱、热分析和单晶X射线衍射技术对其进行了表征。通过吸收光谱、发射光谱和热变性测量研究了这些化合物与CT-DNA的结合特性。通过琼脂糖凝胶电泳探究了这些化合物对超螺旋pBR322质粒DNA的切割活性。还采用XTT法研究了这些化合物对MCF-7、Caco-2、A549癌细胞和BEAS-2B健康细胞的细胞毒性。配合物1和2表现出显著的细胞毒性，其IC50值低于顺铂。
