Carvajal-Arroyo José M, Puyol Daniel, Li Guangbin, Sierra-Álvarez Reyes, Field Jim A
J Biotechnol. 2014 Dec 20;192 Pt A:265-7. doi: 10.1016/j.jbiotec.2014.10.032.
Anammox bacteria are inhibited by nitrite, which is one of their substrates. By utilizing 2,4 dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone, two uncouplers of respiration, we demonstrate that nitrite tolerance of anammox cells is strongly dependent on their ability to maintain a proton gradient, which may be the driving force for active nitrite transport system.
厌氧氨氧化细菌会受到亚硝酸盐的抑制,而亚硝酸盐是它们的底物之一。通过使用两种呼吸解偶联剂2,4-二硝基苯酚和羰基氰化物间氯苯腙,我们证明了厌氧氨氧化细胞对亚硝酸盐的耐受性很大程度上取决于它们维持质子梯度的能力,而质子梯度可能是活性亚硝酸盐转运系统的驱动力。