Li Xiang, Lin Xing, Wang Fan, Yuan Yan, Huang Yong, Yuan Yi, Bi Zhen, Liu Xin, Yang Peng-Bing
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Institute of Environmental Biotechnology, Suzhou University of Science and Technology, Suzhou 215009, China.
Huan Jing Ke Xue. 2017 Jul 8;38(7):2941-2946. doi: 10.13227/j.hjkx.201701163.
The ammonium oxidation by potential electron acceptors(NO, Fe and SO) in ANAMMOX sludge was studied by inoculating nitrite-dependent ANAMMOX sludge. The results showed that nitrite was the most suitable electron acceptor for ANAMMOX bacteria, which could completely react within a short time. Nitrate was converted into nitrite by ANAMMOX sludge using the organic as electron donor and then participated in ammonia conversion, in the absence of nitrite. Fe and SO conversion occurred at the end of ammonium oxidation, but its direct or indirect participation requires further study. Microbial activity must be activated through addition of nitrite as electron acceptor before ammonia excess oxidation occurred. Aerobic ammonia oxidizing bacteria and nitrite oxidizing bacteria grew during this period. This phenomenon did not last, and the microbial production of HO was speculated. Although the oxidation rate was slow, the phenomenon of ammonium excess oxidation was obvious. Therefore, the oxidation of excess ammonia was for sure present in ANAMMOX sludge. The order of electron acceptor use by ANAMMOX sludge was NO, NO, SO and Fe.
通过接种亚硝酸盐依赖型厌氧氨氧化污泥，研究了厌氧氨氧化污泥中潜在电子受体（NO、Fe和SO）对铵的氧化作用。结果表明，亚硝酸盐是厌氧氨氧化细菌最合适的电子受体，能在短时间内完全反应。在没有亚硝酸盐的情况下，厌氧氨氧化污泥利用有机物作为电子供体将硝酸盐转化为亚硝酸盐，然后参与氨的转化。Fe和SO的转化发生在铵氧化的末期，但其直接或间接参与情况有待进一步研究。在氨过度氧化发生之前，必须通过添加亚硝酸盐作为电子受体来激活微生物活性。在此期间，好氧氨氧化细菌和亚硝酸盐氧化细菌生长。这种现象没有持续，推测有微生物产生HO。虽然氧化速率较慢，但铵过度氧化现象明显。因此，厌氧氨氧化污泥中肯定存在过量氨的氧化。厌氧氨氧化污泥使用电子受体的顺序为NO、NO、SO和Fe。
