[Nucleotide sequence analysis for a new HLA-B allele HLA-B*13:01:06*].

[Nucleotide sequence analysis for a new HLA-B allele HLA-B13:01:06].

作者信息

Xiong Baiyu, Tan Yin, Huang Yingfeng, Yang Shaoyu, Luo Hongtu, Shen Liang, Zhou Taichen, Xiao Cangjun

机构信息

Department of Surgery, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical University, Guangzhou, Guangdong 510180, P. R. China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014 Dec;31(6):790-2. doi: 10.3760/cma.j.issn.1003-9406.2014.06.025.

DOI:10.3760/cma.j.issn.1003-9406.2014.06.025

PMID:25449091

Abstract

OBJECTIVE

To confirm a new allele HLA-B*13:01:06 and analyze its nucleotide sequence.

METHODS

Genomic DNA was extracted using a Qiagen DNA extraction kit. Nucleotide sequences of HLA-A, HLA-B, HLA-C and HLA-DRB1 were analyzed by polymerase chain reaction-sequence based typing (PCR-SBT). HLA high-resolution results were assigned, and the nucleotide sequences of HLA-B locus was compared with that of HLA-B*13:01:01.

RESULTS

The nucleotide sequence of the new allele shows a strong similarity to that of HLA-B*13:01:01. One nucleotide in exon 2 has changed from G to A at position 219 (codon 49 GCG>GCA), which however did not result in amino acid change.

CONCLUSION

The novel allele verified by sequencing has been submitted to GenBank and officially named as HLA-B*13:01:06 by the World Health Organization HLA Nomenclature Committee.

摘要