Tsushima Yuko, Uno Naoki, Sasaki Daisuke, Morinaga Yoshitomo, Hasegawa Hiroo, Yanagihara Katsunori
Clinical Laboratory of Nagasaki University Hospital, Nagasaki 852-8501, Japan.
Clinical Laboratory of Nagasaki University Hospital, Nagasaki 852-8501, Japan; Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan.
J Virol Methods. 2015 Feb;212:76-9. doi: 10.1016/j.jviromet.2014.10.019. Epub 2014 Nov 22.
Influenza virus infection is diagnosed in most cases using a rapid influenza antigen diagnostic test (RIDT). However, false-negative results are a major concern. By contrast, the nucleic acid amplification test offers high sensitivity and therefore can aid the interpretation of negative RIDT results. In this study, influenza viral loads were quantified with quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using viral suspensions left over after RIDT, and the performance of both methods was evaluated. qRT-PCR detected as few as 10(3)copies/mL of influenza viruses A and B, whereas RIDT showed negative results for viral loads less than 10(7) and 10(5)copies/mL of influenza viruses A and B, respectively. These results indicate that small quantities of the virus that cause false-negative RIDT results can be detected efficiently with qRT-PCR follow-up. In addition, influenza A virus subtype was determined using qRT-PCR.
大多数情况下，流感病毒感染是通过快速流感抗原诊断检测（RIDT）来诊断的。然而，假阴性结果是一个主要问题。相比之下，核酸扩增检测具有高灵敏度，因此有助于解读RIDT的阴性结果。在本研究中，使用RIDT后剩余的病毒悬液，通过定量逆转录-聚合酶链反应（qRT-PCR）对流感病毒载量进行定量，并评估了两种方法的性能。qRT-PCR能检测到低至10³拷贝/毫升的甲型和乙型流感病毒，而RIDT分别对低于10⁷和10⁵拷贝/毫升的甲型和乙型流感病毒载量显示阴性结果。这些结果表明，通过qRT-PCR后续检测可以有效地检测到导致RIDT假阴性结果的少量病毒。此外，使用qRT-PCR确定了甲型流感病毒的亚型。
