LRIG1通过抑制胶质母细胞瘤中的BCL-2和锰超氧化物歧化酶提高化疗敏感性。

LRIG1 improves chemosensitivity through inhibition of BCL-2 and MnSOD in glioblastoma.

作者信息

Ding Jianjun, Liu Baohui, He Yi, Yuan Xianhou, Tian Daofeng, Ji Baowei, Wang Long, Wu Liquan, Dong Huimin, Wang Junmin, Zhu Xiaonan, Cai Qiang, Zhang Shenqi, Chen Qianxue

机构信息

Zhongnan Hospital, Wuhan University, 125 Donghu Street, Wuhan, 430071, Hubei, China.

出版信息

Cell Biochem Biophys. 2015 Jan;71(1):27-33. doi: 10.1007/s12013-014-0139-6.

DOI:10.1007/s12013-014-0139-6

PMID:25449296

Abstract

We have reported that Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) can improve the chemosensitivity in U251 cells. However, the underlying mechanisms how LRIG1 improves the chemosensitivity remain unknown. In this study, we reported that LRIG1 can improve the chemosensitivity through the inhibition of B cell lymphoma 2 (BCL-2) and manganese superoxide dismutase (MnSOD). In addition, we showed that the expression level of LRIG1 was significantly negatively correlated with BCL-2 and MnSOD expression in glioma. Our research demonstrated that overexpression of LRIG1 can enhance the chemosensitivity. BCL-2 and MnSOD were inhibited in LRIG1 overexpressing cells. On the other hand, when BCL-2 and MnSOD were knocked down, the chemosensitivity of U251 cells decreased, and the effect of LRIG1 in regulating chemosensitivity was compromised. For the first time, our results showed that LRIG1 can enhance chemosensitivity in glioblastoma by inhibition of BCL-2 and MnSOD.

摘要

我们曾报道富含亮氨酸重复序列和免疫球蛋白样结构域1（LRIG1）可提高U251细胞的化学敏感性。然而，LRIG1提高化学敏感性的潜在机制仍不清楚。在本研究中，我们报道LRIG1可通过抑制B细胞淋巴瘤2（BCL-2）和锰超氧化物歧化酶（MnSOD）来提高化学敏感性。此外，我们发现LRIG1的表达水平与胶质瘤中BCL-2和MnSOD的表达显著负相关。我们的研究表明，LRIG1的过表达可增强化学敏感性。在LRIG1过表达的细胞中，BCL-2和MnSOD受到抑制。另一方面，当BCL-2和MnSOD被敲低时，U251细胞的化学敏感性降低，且LRIG1调节化学敏感性的作用受到损害。我们的结果首次表明，LRIG1可通过抑制BCL-2和MnSOD增强胶质母细胞瘤的化学敏感性。