Understanding plant cell morphogenesis requires real-time monitoring of cell wall polymers.

Plant cell development and growth are determined by the expansion pattern of the cell wall, a matrix of mixed polysaccharide polymers and proteins. To understand the different roles of these polymers in the regulation of the morphogenetic process, their spatial dynamics need to be monitored over time. Recent developments in the live cell labeling of polysaccharides include specific dyes whose insertion into the wall does not interfere with wall properties and growth, as well as metabolically inserted labeling. The present review explains the motivation and necessity for novel polysaccharide labeling techniques and provides an overview of the insight gained with these strategies.