Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Rue Sherbrooke est, Montréal, Québec H1X 2B2, Canada.
Institut de recherche en biologie végétale, Département de sciences biologiques, Université de Montréal, 4101 Rue Sherbrooke est, Montréal, Québec H1X 2B2, Canada.
Curr Opin Plant Biol. 2015 Feb;23:76-82. doi: 10.1016/j.pbi.2014.11.007. Epub 2014 Nov 22.
Plant cell development and growth are determined by the expansion pattern of the cell wall, a matrix of mixed polysaccharide polymers and proteins. To understand the different roles of these polymers in the regulation of the morphogenetic process, their spatial dynamics need to be monitored over time. Recent developments in the live cell labeling of polysaccharides include specific dyes whose insertion into the wall does not interfere with wall properties and growth, as well as metabolically inserted labeling. The present review explains the motivation and necessity for novel polysaccharide labeling techniques and provides an overview of the insight gained with these strategies.
植物细胞的发育和生长取决于细胞壁的扩展模式，细胞壁是一种混合多糖聚合物和蛋白质的基质。为了了解这些聚合物在形态发生过程中的不同作用，需要随时间监测它们的空间动态。多糖的活细胞标记的最新进展包括特定的染料，其插入细胞壁不会干扰细胞壁的性质和生长，以及代谢插入标记。本综述解释了新型多糖标记技术的动机和必要性，并概述了这些策略获得的见解。
