Gyllenhammar H
Department of Medicine 3, Karolinska Institute at Södersjukhuset, Stockholm, Sweden.
Scand J Clin Lab Invest. 1989 Jun;49(4):317-22. doi: 10.3109/00365518909089103.
When neutrophils were stimulated with leukotriene B4 (LTB4) .O2- -production and elevation of intracellular Ca2+ were initiated more rapidly than after stimulation with N-formyl-methionyl-leucyl-phenylalanine. Phorbol myristate acetate (PMA) induced a substantially more protracted superoxide (.O-2) production. Luminol augmented chemiluminescence (LCL) reflected the kinetic characteristics accurately. The primary distinguishing attribute of LTB4-induced responses were an initial high rate of activation and a rapid termination. Thus, LTB4-induced neutrophil Ca2+-inflow and .O2- -formation may be initiated by mechanisms similar in part to those for formylpeptides; however it does not possess the ability to maintain this activation. The kinetic properties of neutrophil activation may be studied with LCL, irrespective of the exact nature of the LCL-generating oxidants formed by neutrophils.
当中性粒细胞受到白三烯B4（LTB4）刺激时，与受到N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸刺激相比，超氧阴离子（.O2-）的产生和细胞内Ca2+浓度的升高起始得更快。佛波酯（PMA）诱导产生的超氧化物（.O-2）持续时间长得多。鲁米诺增强的化学发光（LCL）准确地反映了动力学特征。LTB4诱导反应的主要区别特征是初始激活速率高且迅速终止。因此，LTB4诱导的中性粒细胞Ca2+内流和.O2-形成可能部分通过与甲酰肽类似的机制启动；然而，它不具备维持这种激活的能力。无论中性粒细胞形成的产生LCL的氧化剂的确切性质如何，均可通过LCL研究中性粒细胞激活的动力学特性。
