Sobroza Ânderson O, Tonin Alexandre A, Da Silva Alekandro S, Dornelles Guilherme L, Wolkmer Patrícia, Duarte Marta M M F, Hausen Bruna S, Sangoi Manuela B, Moresco Rafael N, Stefani Lenita M, Mazzantti Cinthia M, Lopes Sonia T A, Leal Marta L R
Department of Small Animal, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Department of Small Animal, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Comp Immunol Microbiol Infect Dis. 2014 Dec;37(5-6):299-304. doi: 10.1016/j.cimid.2014.09.003. Epub 2014 Oct 8.
The aim of this study was to analyze the classic iron markers associated to the storage process in hamsters experimentally infected by Leptospira interrogans serovar Pomona. Four groups with six hamsters each were used; two were negative controls (C7 and C14) and two were composed by infected animals (T7 and T14). Blood samples were collected on the seventh (C7 and T7) and fourteenth days (C14 and T14) post-inoculation. Iron availability was determined in sera samples through the assessment of iron, ferritin, transferrin, and iron binding capacity, whereas the bone marrow was also evaluated for the presence of iron by Pearl's reaction. Additionally, the total antioxidant capacity (TAC) and total oxidant status (TOS) were assessed, along with hepcidin and IL-6 levels. Based on the results, it was possible to observe the onset of an anemic profile, predominantly hemolytic and regenerative. Also, The other parameters showed an increase in seric iron (P<0.01) and ferritin (P<0.01), and a positive Pearl's reaction in T7 and T14, when compared with the control groups. Transferrin levels decreased (P<0.05) in animals of T14 with saturation index. TAC was increased in both periods (P<0.01), while TOS was increased only on T14 (P<0.05). Hepcidin and IL-6 were increased on T7 and T14 (P<0.01). Therefore, it was observed that the serum profile from infected animals showed a strong hemolytic pattern, with some demonstration of ferric tissue sequestration when the infection tended to become chronic. The results show that iron metabolism is activated in hamsters infected by L. interrogans serovar Pomona.
本研究旨在分析与实验感染问号钩端螺旋体波摩那血清型的仓鼠储存过程相关的经典铁标志物。使用了四组，每组六只仓鼠；两组为阴性对照组（C7和C14），两组由感染动物组成（T7和T14）。在接种后第七天（C7和T7）和第十四天（C14和T14）采集血样。通过评估铁、铁蛋白、转铁蛋白和铁结合能力来测定血清样本中的铁可用性，同时也通过珀尔反应评估骨髓中铁的存在情况。此外，还评估了总抗氧化能力（TAC）和总氧化状态（TOS），以及铁调素和白细胞介素-6水平。根据结果，可以观察到贫血特征的出现，主要是溶血性和再生性的。此外，与对照组相比，其他参数显示血清铁（P<0.01）和铁蛋白（P<0.01）增加，T7和T14的珀尔反应呈阳性。T14组动物的转铁蛋白水平随饱和指数降低（P<0.05）。两个时期的TAC均增加（P<0.01），而TOS仅在T14增加（P<0.05）。铁调素和白细胞介素-6在T7和T14增加（P<0.01）。因此，观察到感染动物的血清特征显示出强烈的溶血模式，当感染趋于慢性时，有一些铁组织螯合的表现。结果表明，感染问号钩端螺旋体波摩那血清型的仓鼠铁代谢被激活。
